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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2013_09_18 to 2013_11_22
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well documented study according to OECD guideline and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): FC-131
- Substance type: yellow powder
- Physical state: solid
- Analytical purity: 99. 8 % (HPLC)
- Impurities (identity and concentrations): 0.1 %, 4-diethylamino-2-methoxy-benzaldehyde
- Purity test date: 2013_07_03 (certificate of analysis)
- Lot/batch No.: 30610045
- Expiration date of the lot/batch: 2015_06_12
- Storage condition of test material: room temperature (20±5 °C)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic, adapted
Details on inoculum:
- Source of inoculum: sampled at 2013_09_19 from secondary effluent of the biologic sewage treatment plant Borsdorf district Panitzsch (Saxony, Germany)
- Preparation of inoculum for exposure: pre-condition by aerating for 4 days at test temperature, stopped one hour before starting the test to settle coarse particles down; supernatant used as inoculum
- Concentration: 1 mL/L
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
2 mg/L
Based on:
ThOD/L
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Remarks:
in the range: 0 to 28 days
Details on study design:
TEST CONDITIONS
- Medium:analytical grade reagents for mineral medium

- Carrier for test substance: quartz powder, inclusion of carrier control
- Test temperature: 20.5 ± 0.5 °C
- pH adjusted:yes to 7.4 ± 0.1 (in stock solution)
- Continuous darkness: yes


TEST SYSTEM
- Culturing apparatus: glass bottles with glas stoppers, 250 mL
- Number of culture flasks/concentration/condition: 3
- Method used to create aerobic conditions:aeration of sewage (inoculum) before test
- Measuring equipment: Oximeter with an O2-electrode and pH-meter with pH-electrode (Labmeter Hach HQ440d multi)


CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Carrier blank: Yes
- Toxicity control: Yes
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

Test performance:
The highest degree of degradation observed was 4.7 % (without considering nitrification).

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
2.4
Sampling time:
4 d
Remarks on result:
other: this values are without nitrification
Parameter:
% degradation (O2 consumption)
Value:
2.9
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
4.7
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
3.5
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
0.8
Sampling time:
28 d
Details on results:
The results of the calculated pecentage degradation with complete nitrification are comparable with the results in the table above (without nitrification). The toxicity control indicated no toxicity of the test item towards the inoculum.

BOD5 / COD results

Results with reference substance:
For the reference substance sodium acetate 81.7 % degradation was observed on day 14.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
As degradation was much below 60 %, the test item FC-131 is considered as not ready biodegradable .
Executive summary:

The areobic degradation potential of the test item FC-131 was investigated using the Closed Bottle test according to the OECD guideline No 301 D and GLP. The used inoculum was sampled in a biologic sewage treatment plant. The test was performed for 28 days. The maximum degradation rate observed was 4.7 %. Hence, FC-131 is considered as not ready biodegradable. The study is considered valid and reliable.