Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Batch number: Charge 825
- Date of production: 22 Sep 1999
- Molecular formular: no details
- Molecular weight [g/mol]: no details
- Aggregate state: liquid
- Colour: colourless
- Purity of the test substance: 99.78 area-%
- Impurities: no details
- Homogeneity: yes

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage.
- Preparation of inoculum for exposure: inoculum was washed and aerated for 24 hours before dosing into the test vessels
- Pretreatment: none
- Concentration of sludge: 30 mg/l dry substance
Duration of test (contact time):
34 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: room temperature
- pH: 7.2-7.5


SAMPLING
- Sampling frequency: continuously for 28 days


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
- Other: inhibition of the inoculum
Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
30 - 40
Sampling time:
28 d
Parameter:
% degradation (O2 consumption)
Value:
40 - 50
Sampling time:
34 d
Details on results:
- Duration of adaption phase: 13 days
- Duration of degradation phase: 12 days

- Degradation of reference substance after 14 days (BOD/ThOD): 70-80%

Applicant's summary and conclusion

Interpretation of results:
other: partly or moderately biodegradable and not readily biodegradable according to OECD criteria