Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 February 2011 – 27 April 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This study has been performed according to OECD and EC guidelines and in compliance with GLP principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report Date:
2011

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)
Version / remarks:
(1995)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient)
Version / remarks:
(Official Journal of the European Union no. L142, May 31, 2008)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
shake-flask method to: flask method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): E-Y110
- Test substance storage: At room temperature protected from light
- Stability under storage conditions: Stable
- Description: Orange powder
- Batch: MB-1
- Expiry date: 23 December 2015

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficientopen allclose all
Type:
Pow
Partition coefficient:
< 0.001
Temp.:
20 °C
pH:
7.1
Type:
log Pow
Partition coefficient:
< -3.3
Temp.:
20 °C
pH:
7.1
Details on results:
The log Pow values were within the criterion range of ± 0.3 log units.

A small peak was detected in the pretreated samples from the blank mixture. The maximum contribution to the samples was 1.6%.

Any other information on results incl. tables

The results of the shake flask method are given in the Table below:

Main study – Powof the test substance

Ratio

n-octanol : buffer pH 7

 [v:v]

Analysed concentration

Pow 

Log Pow

pH

n-octanol

[g/l]

buffer pH 7

[g/l]

individual

1:1

 

 1:2

 

 

2:1

< 0.000585

< 0.000587

 

< 0.000580

< 0.000592

 

< 0.000582

< 0.000590

0.718

0.713

 

0.552

0.540

0.967

1.06

< 8.14 x 10-4

< 8.24 x 10-4

 

< 1.05 x 10-3

< 1.10 x 10-3

 

< 6.02 x 10-4

< 5.58 x 10-4

< -3.1

< -3.1

 

< -3.0

< -3.0

 

< -3.2

< -3.3

7.1

7.1

 

7.1

7.1

 

7.1

7.1

The results on the recoveries of the test substance in the test samples are given in the Table below. The analysed concentration of the stock solution was 2.04 g/l. Based on this the nominal quantity of the test substance in the test samples was 4.08 mg.

Recoveries

Ratio

n-octanol : buffer pH 7

 

[v:v]

Analysed quantity

Recovery

 

 

[%]

n-octanol

[mg]

buffer pH 7

[mg]

Total

[mg]

1:1

 

 

1:2

 

 

2:1

< 0.00351

< 0.00352

 

< 0.00232

< 0.00237

 

< 0.00465

< 0.00472

4.31

4.28

 

4.41

4.32

 

3.87

4.23

4.31

4.28

 

4.42

4.32

 

3.87

4.23

106

105

 

108

106

 

95

104

Applicant's summary and conclusion

Conclusions:
The shake flask method was applied for the determination of the partition coefficient (Pow) of E-Y110. The Pow value of the test substance at neutral pH and 20°C was < 5.58 10^-4, which corresponds with a log Pow value of < -3.3.