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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl 5-oxo-1-phenyl-2-pyrazoline-3-carboxylate
EC Number:
201-899-4
EC Name:
Ethyl 5-oxo-1-phenyl-2-pyrazoline-3-carboxylate
Cas Number:
89-33-8
Molecular formula:
C12H12N2O3
IUPAC Name:
ethyl 5-oxo-1-phenyl-4,5-dihydro-1H-pyrazole-3-carboxylate
Details on test material:
purity: 99.7 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Length at study initiation: 2.5 - 3.5 cm average 3.0 cm s= 0.28 cm
- Feeding during test: no
ACCLIMATION
- Acclimation period: about 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice a day ad libitum before

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Test temperature:
21 - 23 °C
pH:
5.7 - 8.1
Dissolved oxygen:
7.4 - 8.8 mg/L
Nominal and measured concentrations:
Nominal concentrations: 0, 180, 250, 350, 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass container (30cm x 22cm x 24cm)
- Type (delete if not applicable): open / closed
- Fill volume: 10 liters
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: composition according to ISO/DIS 7346/1


OTHER TEST CONDITIONS
- Photoperiod: 12 hours
- Light intensity: 700 Lux

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
250 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
350 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: LC90
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 350 - < 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities: projecting opercula, sometimes increased respiration rate, sometimes hypoactivity, sometimes increased fright reaction.
In the group with 180 mg/L the fish showed no more symptoms after 48 hours.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC0 is 350 mg/L and LC50 between 350 and 500 mg/L. Therefore the substance is not subject for classification concerning the toxicity to fish.
Executive summary:

The substance was tested for toxicity to fish (Zebrabärbling) according to OECD guideline 203. The test design was devided in three groups: a: 0 and 180 mgL, b: 0 and 500 mg/L, c: 0, 250 and 350 mg/L. For each test 10 fish were used. After 96 hours only the test group with 500 mg/L showed letality (9/10) and symptoms. The groups with 180, 250 and 350 mg/L showed symptoms but no letality.

The substance is only poorly soluable in water, therefore residual undissolved material could be observed at the ground of the test container.