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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24.10. - 8.11. 2013
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Meets national standard method (ČSN EN ISO).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
ISO 6341 15 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Deviations:
no
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,1,1,2,3-pentachloropropane
- Substance type: organic
- Physical state: liquid
- Analytical purity: 99.940 % (w/w)
- Impurities (identity and concentrations): chlorinated hydrocarbons - 0.048 % (w/w)
- Lot/batch No.: 2-750
- Expiration date of the lot/batch: august 2014
- Storage condition of test material: dry place at room temperature

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The stock solution of the test substance was prepared in dilution water. 20 mg of the test substance was weighed into 200 mL of the dilution water for the preliminary test and 200 mg of the test substance was weighed into 2000 mL of the dilution water for the full test. The concentrations of solutions used in the preliminary and full test were obtained by dilution of the stock solution with dilution water.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: own laboratory breeding
- Age at study initiation (mean and range, SD): max. 24 hours
- Method of breeding: aperiodic parthenogenesis
- Feeding during test: no

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
sum of Ca + Mg ions: cca 2.5 mmol/L
Test temperature:
20 ± 2°C
pH:
7.5 - 7.9
Dissolved oxygen:
7.5 - 8.6 mg/L
Nominal and measured concentrations:
Nominal concentration:
- Preliminary test: 1, 5, 10, 50 and 100 mg/L
- Full test: 56, 62, 68, 75, 83, 91 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: beaker
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
Standard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1000 mL of deionized water.
Solution A: 117.6 g CaCl2·2H2O in 1 L of deionized water
Solution B: 49.3 g MgSO4·7H2O in 1 L of deionized water
Solution C: 25.9 g NaHCO3 in 1 L of deionized water
Solution D: 2.3g KCl in 1 L of deionized water
pH 7.8 ± 0.2
Sum of Ca + Mg ions: approx.2.5 mmol/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: natural cycle of light and dark
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
77.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 60.0 - 113.9 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
82.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 68.3 - 215.2 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
< 56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
< 56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
POSITIVE CONTROL
The sensitivity of the test species and correctness of test performance was verified by testing with the reference substance, potassium dichromate: EC50 (48 hour) = 0.83 mg/L

INTERLABORATORY TEST DATA
From last interlaboratory test arranged by CSlab, s.r.o. in 2013 was obtained data for potassium dichromate 48h – EC50 generated at 13 laboratories. The following reference range of values could be calculated from those data:
EC50 (48 hour) = 0.58 – 1.34 mg/L

METHOD VALIDITY EVALUATION
The immobilisation of test organism by the reference substance (48h – EC50) obtained from reference test meets the calculated range from the interlaboratory test. The sensitivity of test species was proved.

Any other information on results incl. tables

Table No. 1: Results and conditions of preliminary and full test

Preliminary test

Nominal concentration

mg/L

100

50

10

5

1

control

Number of mobile animals

24 hours

0

10

10

0

10

10

Immobilisation (%)

24 hours

100

0

0

100

0

0

Number of mobile animals

48 hours

0

10

10

0

10

10

Immobilisation (%)

48 hours

100

0

0

100

0

0

O2 (mg/L)

0 hour

7.9

7.9

7.7.

7.9

7.9

7.5

O2 (mg/L)

48 hours

8.0

7.7

8.1

7.8

7.9

7.9

pH

0 hour

7.7

7.6

7.7

7.7

7.7

7.8

pH

48 hours

7.7

7.6

7.7

7.7

7.7

7.8

Full test

Nominal concentration

mg/L

100

91

83

75

68

62

56

control

Number of mobile animals

24 hours

1stseries

0

5

5

8

9

9

8

10

Number of mobile animals

24 hours

2ndseries

0

5

7

7

8

7

9

10

Number of mobile animals

24 hours

total

0

10

12

15

17

16

17

20

Immobilisation (%)

24 hours

100

50

40

25

15

20

15

0

Number of mobile animals

48 hours

1stseries

0

5

5

7

9

8

7

10

Number of mobile animals

48 hours

2ndseries

0

4

6

4

5

6

8

10

Number of mobile animals

48 hours

total

0

9

11

11

14

14

15

20

Immobilisation (%)

48 hours

100

55

45

45

30

30

25

0

O2 (mg/L)

0 hour

8.6

8.6

8.6

8.6

8.6

8.6

8.6

8.3

O2 (mg/L)

48 hours

8.0

8.0

7.9

8.2

8.0

8.2

7.9

7.9

pH

0 hour

7.8

7.8

7.8

7.8

7.8

7.8

7.8

7.8

pH

48 hours

7.5

7.5

7.5

7.5

7.5

7.5

7.5

7.5

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The determination of harmful effects of the test substance 1,1,1,2,3 - pentachloropropane exerted in short time exposure resulting in the immobilisation of daphnia. Acute toxicity is expressed as the median effective concentration EC50. Daphnia magna, Acute Immobilisation Test was performed according to the standard ČSN EN ISO 6341 (75 7751), Jakost vod – Zkouška inhibice pohyblivosti Daphnia magna Straus (Cladocera, Crustacea) – Zkouška akutní toxicity, 1997. The mentioned testing procedure is accredited as test method T18 in the testing laboratory.
The values of EC50 were calculated from equation of regression line. The NOEC and EC100 values are identified by direct observation.
Test results (nominal concentration):
EC50 (24 hour) = 82.8 mg/L (95% confidence limit: 68.3 – 215.2 mg/L)
EC50 (48 hour) = 77.2 mg/L (95% confidence limit: 60.0 – 113.9 mg/L)
NOEC (24 hour) < 56 mg/L
NOEC (48 hour) < 56 mg/L
EC100 (24 hour) = 100 mg/L
EC100 (48 hour) = 100 mg/L
Executive summary:

The determination of harmful effects of the test substance 1,1,1,2,3 - pentachloropropane exerted in short time exposure resulting in the immobilisation of daphnia. Acute toxicity is expressed as the median effective concentration EC50. Daphnia magna, Acute Immobilisation Test was performed according to the standard ČSN EN ISO 6341 (75 7751), Jakost vod – Zkouška inhibice pohyblivosti Daphnia magna Straus (Cladocera, Crustacea) – Zkouška akutní toxicity, 1997. The mentioned testing procedure is accredited as test method T18 in the testing laboratory.

The values of EC50were calculated from equation of regression line. The NOEC and EC100values are identified by direct observation.

Test results (nominal concentration):

EC50(24 hour) = 82.8 mg/L (95% confidence limit: 68.3 – 215.2 mg/L)

EC50(48 hour) = 77.2 mg/L (95% confidence limit: 60.0 – 113.9 mg/L)

NOEC (24 hour) < 56 mg/L

NOEC (48 hour) < 56 mg/L

EC100(24 hour) = 100 mg/L

EC100(48 hour) = 100 mg/L