Bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline and GLP compliant study

Data source

Reference

Reference Type:

other: Inquiry result from ECHA

Title:

Unnamed

Year:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Directive 84/449/EEC, Method B1 OECD Guideline No. 401

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc

EC Number:

403-360-0

EC Name:

Bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc

Cas Number:

42405-40-3

Molecular formula:

C30H42O6Zn

IUPAC Name:

bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc

Test animals

Species:

other: Rat, Sprague-Dawley

Administration / exposure

Vehicle:

other: 1% Carboxymethylcellulose in distilled water

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 800 mg/kg bw

95% CL:

1 400 - 2 200

Remarks on result:

other: Slope of mortallity curve: 4

Mortality:

Male: 500 mg/kg bw; Number of animals: 7; Number of deaths: 0
Male: 800 mg/kg bw; Number of animals: 5; Number of deaths: 0
Male: 1000 mg/kg bw; Number of animals: 2; Number of deaths: 2
Male: 1260 mg/kg bw; Number of animals: 5; Number of deaths: 3
Male: 2000 mg/kg bw; Number of animals: 10; Number of deaths: 7
Male: 2500 mg/kg bw; Number of animals: 2; Number of deaths: 2
Male: 3200 mg/kg bw; Number of animals: 5; Number of deaths: 2
Male: 5000 mg/kg bw; Number of animals: 10; Number of deaths: 6
Male: 8000 mg/kg bw; Number of animals: 5; Number of deaths: 5

Female: 500 mg/kg bw; Number of animals: 7; Number of deaths: 0
Female: 800 mg/kg bw; Number of animals: 5; Number of deaths: 0
Female: 1000 mg/kg bw; Number of animals: 2; Number of deaths: 1
Female: 1260 mg/kg bw; Number of animals: 5; Number of deaths: 2
Female: 2000 mg/kg bw; Number of animals: 10; Number of deaths: 5
Female: 2500 mg/kg bw; Number of animals: 2; Number of deaths: 1
Female: 3200 mg/kg bw; Number of animals: 5; Number of deaths: 5
Female: 5000 mg/kg bw; Number of animals: 10; Number of deaths: 7
Female: 8000 mg/kg bw; Number of animals: 5; Number of deaths: 5

Clinical signs:

Signs of toxicity related to dose levels:
Mortalities:

Mortalities occured amongst rats dosed at 1260mg/kg and above, within 1 and 47 hours after dosing

Clinical signs:
Signs of reaction to treatment shortly after dosing in all rats were pilo-erection and lethargy.
These were accompanies amongst rats from all groups by hunched posture, abnormal gait, decreased respiratory rate and pallor of the extremities.
Increased salivation was observed in all female rats dosed at 5000mg/kg and two female rats at 8000mg/kg.

A comatose like condition was observed amongst rats dosed at 2000 mg/kg and above.

Gasping was oberved in one female rat dosed at 5000 mg/kg and one female dosed at 8000 mg/kg.

Noisy respiration was observed in one female at 5000 mg/kg and two females at 8000 mg/kg.

Recovery, as judged by external appearance and behaviour, was apparently complete by day 5.

Gross pathology:

Effects on organs:
Autopsy of animals that had died during the study revealed pallor of the kidneys and spleen. Occasional incidences of haemorrhagic lungs, pallor and patchy livers and pale lungs were also observed.

Terminal autopsy findings were normal.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU