Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 939-401-9 | CAS number: 1469983-41-2
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
An Activated Sludge Respiration Inhibition test with the source substance showed that the 3-h NOEC is >=100 mg/L. There was no significant inhibitory effect on the respiration rate of activated sludge.
Key value for chemical safety assessment
Additional information
Executive summary of the study performed with the source substance:
The effect of P-2290 on the respiration rate of activated sludge was assessed by the methods detailed in EC Directive 881302, 'Biodegradation - Activated Sludge Respiration Inhibition test' and OECD Test Guideline 209, 'Activated Sludge, Respiration Inhibition test' adopted 4 April 1984, and the US Environmental Protection Agency (EPA), Toxic Substances Control Act, (TSCA), and the Federal Insecticide, Fungicide, and Rodenticide Act, (FIFRA); Office of Prevention, Pesticides and Toxic Substances, (OPPTS) Method 850.6800, (public draft issued April 1 W6), Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals. Samples of activated sludge (suspended solids 1.6 g/l), fed with synthetic sewage, were exposed to the test substance at nominal concentrations of 1, 10 and 100 mg/L for three hours. Single mixtures were prepared at 1 and 10 mg/L and the highest level was prepared in triplicate. Their rates of oxygen consumption were determined using an oxygen electrode and compared with those of controls, containing activated sludge and synthetic sewage alone, which were established at the beginning and end of the culture series. The reference inhibitor 3,5 -dichlorophenol (3,5 -DCP) was employed at 3.0, 10.0 and 32.0 mg/l, as a positive control. The specific respiration rate of the control culture established at the end of the test series (24.2 mgO2/g/h) was 98% of the rate of that established at the start (24.6 mg02/g/h). The three-hour 50% effect concentration (EC50) for 3,5 -DCP was calculated to be 9.4 mg/l (95% confidence limits 7.4 - 11.8 mg/l). These results show that the test was valid and that the sample of activated sludge employed was sensitive to inhibition. P-2290 had no biologically significant inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. The respiration rate of activated sludge was reduced at most by 10% of the mean control value, in one replicate at a nominal concentration of 100 mg/l; no reduction was seen in two further replicates at this level. The EC20, EC50 and EC100 of the test substance could not therefore be calculated but these must be greater than 100 mg/l, the highest level tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
