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EC number: 407-560-9
CAS number: 107934-68-9
Incidence of Immobility in the Final Test
A study was performed to assess the acute toxicity of the
test item to Daphnia magna. The method followed was designed to be
compatible with the OECD Guidelines for Testing of Chemicals No 202 and
also the Guidance document on aquatic toxicity testing of difficult
substances and mixtures, OECD series on testing and assessment number
23, 2000 under GLP conditions.
Due to the low aqueous solubility and complex nature of the test
item for the purposes of the test the test item was prepared as a Water
Accommodated Fraction (WAF). Twenty daphnids (4 replicates of 5 animals)
were exposed to a Water Accommodated Fraction (WAF) of the test item at
two nominal loading rates of 5 and 100 mg/L for 48 hours at a
temperature of ca. 21 °C under semi-static test conditions. The number
of immobilized Daphnia and any adverse reactions to exposure were
recorded after 24 and 48 hours.
The 48-Hour EL50 for the test item to Daphnia magna based on
nominal loading rates was greater than 100 mg/L loading rate WAF,
equivalent to greater than 1.7 µg/L based on measured concentrations
(EC50). This indicates that, in the range of its water solubility, the
test item was not toxic under the test conditions.
EC50 (48h) > 1.7 µg/L (measured) for Daphnia
magna (OECD 202).
No toxicity observed at the limit of
solubility of the test substance.
A second study from 1991 is available.
However, that second study used a solubilizer to increase the
concentration of the test substance in the medium. The observed effects
on the mobility of Daphnia magna are observed at concentrations
exceeding the solubility of the test material (EC50 1.1 mg/L for a
solubility < 0.052 mg/L). Therefore, this result was considered as
One reliable study is available on the
short-term toxicity of 4,4´-(9H-fluoren-9-ylidene)bis(2-chloroaniline)
(CAS No. 107934-68-9) to aquatic invertebrates. The study was performed
according to OECD guideline 202 and GLP, with water fleas (Daphnia
magna) as test organisms. The daphnids were exposed to the substance for
48 hours at measured test concentrations of 1.3 and 1.7 µg/l (equivalent
to nominal concentrations of 5 and 100 mg/L). Up to the measured
concentration of 1.7 µg/l no effects on the mobility of the test
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