Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-809-5
CAS number: 4500-29-2
The sensitization potential of the test item was investigated using the
maximization method according to Magnusson and Kligman (OECD TG 406) in
guinea pigs in accordance with the principles of GLP. 10 male test and 5
male control animals were used for the study. For selection of the test
concentrations to be used in the main study, irritation screens
following intradermal and topical administration of test substance were
performed. After intradermal injection of 0.1, 0.5, 1 and 5% (v/v) the
degree of erythema was assessed. Due to severe skin reactions the
animals treated with 1% and 5% were killed for humane reasons following
the 24 hour observation. 0.1% causing mild to moderate irritation was
selected for the intradermal induction treatment for the main study.
Screening for topical induction concentrations were performed using 75%,
50%, 25%, 5%, 1%, 0.5% and 0.1% (v/v) test substance in water. Due to
severe dermal reactions the animals treated with 25% and higher were
killed for humane reasons immediately after the 1 hour observation. 5%
test item in water which was tolerated systemically and produced only
mild to moderate dermal irritation was selected for the topical
induction stage of the main study and 5% as well as 1% for the dermal
challenge tretament. Patches for both topical induction and challenge
were kept in contact with the skin under occlusive conditions for 48 and
24 hours respectively. Following the topical induction, discrete or
patchy to moderate erythema was elicited by the test material in all
animals from the test and control group. However, no skin reactions were
noted at the challenge sites (5% and 1%) of the test or control group
animals at the 24 or 48 hour observations. Based on the results, the
test item produced a 0% (0/10) sensitisation rate and is considered to
be a non-sensitiser to guinea pigs under the conditions of the test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again