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EC number: 224-809-5
CAS number: 4500-29-2
Rates of the Definitive Test
after 24 and 48 h of Exposure
= 20, divided into 4 replicates with 5 daphnids each)
In the acute immobilization test with Daphnia
magna (STRAUS) the effects of the test item 2,2’-(Cyclohexylimino)diethanol(batch
number: ESD0011452) were determined according to OECD 202 (2004) from 2011-08-03
to 2011-08-05 at Dr.U.Noack-Laboratorien,
Käthe-Paulus-Str.1, 31157 Sarstedt, Germany
The study was conducted under static
conditions over a period of 48 h with 5concentrations of the test item 2,2’-(Cyclohexylimino)diethanolranging
from 25.0 to 400 mg/L set up in a geometric series with a dilution
factor of 2. Twenty daphnids were exposed to each concentration level,
the pH-control and the control.
The active ingredient 2,2’-(Cyclohexylimino)diethanolwas
analytically verified by LC-MS/MS in the fresh (0 h) and old media (48
h) of all concentration levels, the pH-control and the control.
The recoveries of the active ingredient 2,2’-(Cyclohexylimino)diethanolwere
in the range of 107 to 114 % of the nominal values in the fresh media (0
h) and 101 to 115 % in the old media (0 h)demonstrating
that concentrations of 2,2’-(Cyclohexylimino)diethanolwere
successfully maintained for the duration of the test. The
analytical results are presented in part 4.2.2.
All effect values, presented in Table 1,
are based on the nominal concentrations of the test item.
quality parameters (pH-value and dissolved oxygen concentration),
measured at 0 and 48 h, were determined to be within the acceptable
limits. The validity criteria of the test guideline were fulfilled.
EC50- (with Confidence Interval), EC100 - Values,
NOEC and LOEC
on the nominal concentrations of the test item 2,2’-(Cyclohexylimino)diethanol)
Nominal Concentrationof the Test Item[mg/L]
Confidence Intervalp = 95%[mg/L]
296 - 312
44.0 - 82.4
358 - 372
141 - 192
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