3-(chloromethyl)heptane

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (pre-GLP; application time 20 h; occlusive coverage; only 2 animals used; short observation period (8 days)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Principles of method if other than guideline:

The test substance was applied to a 2.5 x 2.5 cm application site of white Vienna rabbits for 20 h under occlusive conditions. The skin was intact. After the application time, the skin was washed with water which may have contained a mild detergent. Animals were observed for 8 days and skin changes were observed on working days. Findings were recorded after 24 and 48 hours and at the end of the observation period. Findings were graded as described in OECD guideline 404 (according to Draize).

GLP compliance:

no

Remarks:

GLP was not compulsory at the time the study was conducted

Test material

Test material information

Constituent 1

Reference substance name:

Ethylhexylchloride

IUPAC Name:

Ethylhexylchloride

Details on test material:

- Name of test material (as cited in study report): Ethylhexylchloride
- Physical state: liquid
- Purity: ca. 98%

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler
- Age at study initiation: young adult
- Weight at study initiation: animal 1: 2.13 kg; animal 2: 2.21 kg
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin of same animal

Amount / concentration applied:

TEST MATERIAL
- Amount applied (volume): approx. 0.5 ml
- Concentration (if solution): undiluted

Duration of treatment / exposure:

The test substance was applied for 1 min, 5 min, 15 min and 20 h. The irritation scores from the 20-hour exposure period were taken to assess skin irritation by the test substance.

Observation period:

8 days

Number of animals:

2 (males)

Details on study design:

TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm
- Type of wrap if used: occlusive

REMOVAL OF TEST SUBSTANCE
- Washing: yes, with water which may have contained a mild detergent
- Time after start of exposure: 1 min, 5 min, 15 min, 20 h

SCORING SYSTEM: as described in OECD guideline 404 (according to Draize)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

other: 24-48 hours

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: 20 h occlusive exposure, 72-hour assessment timepoint missing, short observation period (8 days)

Results 2

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

other: 24-48 hours

Score:

2.5

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: 20 h occlusive exposure, 72-hour assessment timepoint missing, short observation period (8 days)

Results 3

Irritation parameter:

edema score

Basis:

animal #1

Time point:

other: 24-48 hours

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Remarks on result:

other: squamation

Results 4

Irritation parameter:

edema score

Basis:

animal #2

Time point:

other: 24-48 hours

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Remarks on result:

other: squamation

Irritant / corrosive response data:

See table below.

Other effects:

- Weight at study termination: animal 1: 2.38 kg; animal 2: 2.3 kg

Any other information on results incl. tables

Irritation scores (20 h occlusive exposure):

Timepoint	Animal No.	Intact skin		Symptoms
		Erythema	Edema
24 h	1	3	0
	2	2	0
48 h	1	3	0
	2	3	0
8 d	1	2	0	Squamation
	2	2	0	Squamation
Mean 24, 48 h	1	3	0
Mean 24, 48 h	2	2.5	0

 

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information

Conclusions:

According to the results of this study the test substance is considered to be irritating to the rabbit skin.