Registration Dossier
Registration Dossier
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EC number: 202-549-3 | CAS number: 96-98-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed guideline-conform study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: 92/69IEWG (Teil CA-A),
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-nitro-p-toluic acid
- EC Number:
- 202-549-3
- EC Name:
- 3-nitro-p-toluic acid
- Cas Number:
- 96-98-0
- Molecular formula:
- C8H7NO4
- IUPAC Name:
- 4-methyl-3-nitrobenzoic acid
- Details on test material:
- purity: 99.4 % +- 0.6%
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Source: Domestic waste water treament plant Frankfurt am Main / Sindlingen
KInd of inoculum: centrifuged sediment from activated sludge was washed, centrifuged again and resuspended
Pre-treatment: 6 days aeration - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 55 mg/L
- Based on:
- test mat.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 1 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 7.6
- Sampling time:
- 11 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 8.7
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- day 7: 101.3 % biodegradation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The substance is not readily biodegradable
- Executive summary:
The substance was tested for ready biodegradation according to OECD guideline 301 A.
The rate of biodegradation was below 20% after 28 days.
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