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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
α,α,α,2-tetrachlorotoluene
EC Number:
218-377-7
EC Name:
α,α,α,2-tetrachlorotoluene
Cas Number:
2136-89-2
Molecular formula:
C7H4Cl4
IUPAC Name:
1-chloro-2-(trichloromethyl)benzene
Details on test material:
Purity: 97.0 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test item was mixed with water at 30°C and homogenised using an Ultra-Turrax and by ultrasonic treatment.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Strain: Hamilton-Buchanan
- Source: West Aquarium Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): 3 months
- Length at study initiation (mean, range and SD): 2.8 cm, 2.4 - 3.4 cm, 0.20 cm
- Method of breeding: 22 +- 1 °C; O2-content >= 80% ; 12 h day/night cycle, 700 Lux; <= 1g fish/l water;
- Feeding during test: no feeding during test

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Type and amount of food:
- Food type: Tetra Min, Tetra Werke, Melle, Germany;
- Amount: ad libitum;
- Frequency: 2 times a day;

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Test temperature:
Test groups: 21.0 - 22.8 °C
Control groups: 21.6 - 22.7 °C
pH:
Test groups: 6.4 - 8.0
Control groups: 7.3 - 8.1
Dissolved oxygen:
Test groups: 6.2 - 8.8 mg/l
Control groups: 7.0 - 9.3 mg/l
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
350 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
350 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
>= 500 - <= 710 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 500 - <= 710 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
710 mg/L
Nominal / measured:
nominal
Details on results:
A probit analysis to determine the LC50 was not possible given the distribution of the effective concentrations and the dilution factors used.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
From this study, LC0 (48 h and 96 h) for 2-chlorobenzotrichloride was determined to be 350 mg/L, LC50 (48 h and 96 h) to be 500 - 710 mg/L and LC100 (496 h) to be 710 mg/L.