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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Method C.2 Acute toxicity for Daphnia Directive 67/548/EEC, Annex V, Part C: Methods for the determination of ecotoxicity. Published in OJ No L 383 A 1992.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
463-070-5
EC Name:
-
Cas Number:
106966-25-0
Molecular formula:
C7H17N2O2Br
IUPAC Name:
2-(3-methoxy-3-oxopropyl)-1,1,1-trimethylhydrazinium bromide

Test solutions

Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: No auxiliary solvent.
Test solution: dilution water
Standard dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D in 1000 mL of distilled water.
A. 117.6 g CaCl2 . 2 H2O in 1 L distilled water
B. 49.3 g MgSO4 . 7 H2O in 1 L distilled water
C. 25.9 g NaHCO3 in 1 L distilled water
D. 2.3 g KCl in 1 L distilled water
The dilution water should be of pH 7.8 ± 0.2. It is satured by oxygen by the aeration attemperature of 20-24 degree of Celsius. The content of sum Ca + Mg ions should have the value of 2.5 mmol/L.

Test organisms

Test organisms (species):
other aquatic crustacea: Daphnia Magna Straus

Study design

Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
260 mg CaCO3/L
Test temperature:
19.1 degree of Celsius
pH:
pH of dillution water : 7.7
Dissolved oxygen:
8.9 mg/L at temperature of 19.1 degree of Celsius
Nominal and measured concentrations:
Full test:
test substance concentrations: 120, 100, 64, 32, 16, 8 and 4 mg/L
Details on test conditions:
Temperature: 20 ± 2 degree of Celsium, with variatim of 1 degree of Celsius at each individual test
Exposure time: 48 hours
Lighting: daylight
Number of test animals: 20 for each concentration and control in preliminary test, 20 for each concentration and control in full test
Volume of test substance solution: 50 mL (5 mL per individual)
Concentration of dissolved oxygen: higher than 3 mg/L
Without feeding, without aeration.
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
52.8 mg/L
Nominal / measured:
nominal
Remarks on result:
other: 39.7-65.9 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
120 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
8 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Details on results:
%Concentration loss over test: 0 ... 5.3

Applicant's summary and conclusion

Conclusions:
The test substance is harmful for aquatic organisms -daphnia.