α,α,α-trifluorotoluene

Short-term toxicity to fish

Description of key information

The LC50 to Oryzias latipes after 96-h exposure period was determined to be 19.4 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

19.4 mg/L

Additional information

A study on rice fish (Oryzias latipes) was published by the Ministery of Environment, Japan. A study was conducted according to Methods of Testing New Chemical Substances (Algae growth inhibition test, Daphnia acute immobilization test, and Fish acute toxicity test) (Yakushokuhatsu No.1121002, Heisei 15・11・13 Seikyoku No.2, Kampo Kihatsu No. 031121002, 2003). 10 fish per test group were tested in a semi-static test setup with renewal of the medium every 24 hours. Five test concentration 2.50, 5.00, 10.0, 20.0, 40.0 mg/L, and a control were run. The concentration of the test item in the test solutions was determined with Gas chromatography - mass spectrometry (GC/MS). The percentage of measurement concentration to nominal concentration was 66 to 78 % at test solution preparation and 64 to 77 % after 24 hours. It was assumed that the test substance volatilized from water during the test solution preparation, whereas the volatilization from the water surface was prevented by PTFE sheet during the test. After 96 hours of exposure the LC50 was determined to be 19.4 mg/L, the LC0 was determined to be 7.07 mg/L and the LC100 to be 31.0 mg/L.

In a bioconcentration study with the test item an scute toxicity test with Medaka fish (Oryzias latipes var.) was conducted by the Ministery of Environment, Japan. Fish were exposed to the test item for 48 hours in a semi-static setup with medium renewal verey 8 to 16 hours. The LC50 after 48 hours was determined to be 28 mg/L.