Acrylate of bisphenol A-type epoxy resin

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 12 August 2019 to 6 September 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

- Common name: Pseudokirchneriella subcapitata
- Strain: 278/4
- Source (laboratory, culture collection): SAMS Limited
- Method of cultivation: Freshwater with nutrient medium (3N=BBM+V) was autoclaved at 120 ºC for 15 minutes. Pre-cultures in the exponential growth phase were then prepared from stock laboratory cultures by inoculating the freshwater 2 to 3 days before test commencement.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

21 – 24 ºC 2 °C

pH:

7.5 ±0.1

Nominal and measured concentrations:

Nominal concentrations: 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL capacity borosilicate glass crystallising dishes
- Type: closed
- Volume of solution: 50 mL
- Aeration: No aeration during the 72 hour study
- Type of flow-through: static
- Initial cells density: initial cell density of approximately 494.5 x 10^4 cells per millilitre
- No. of vessels per concentration (replicates): three
- No. of vessels per control (replicates): six

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Light intensity and quality: 4440 to 8880 lx

TEST CONCENTRATIONS
- Test concentrations: 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L (definitive test)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

> 1 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- 72h EC50: 1.503 mg/L

Reported statistics and error estimates:

The result is based on nominal concentrations and was calculated by non-linear regression within the CETIS suite of statistical analysis

Validity criteria fulfilled:

yes

Conclusions:

The 72-hour EC50 was >10,000 mg/L and the 72-hour NOEC 1,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Executive summary:

The short-term toxicity of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid (EC 948-054-2) to algae was determined during a GLP-compliant

study performed according to the OECD Testing Guideline 201.

The species was Pseudokirchneriella subcapitata.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

As a result of this range-finding test, concentrations of 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L were used during the definitive test.

The 72-hour EC50 was >10,000 mg/L and the 72-hour NOEC 1,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Description of key information

The short-term toxicity of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid (EC 948-054-2) to algae was determined during a GLP-compliant

study performed according to the OECD Testing Guideline 201.

The species was Pseudokirchneriella subcapitata.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

As a result of this range-finding test, concentrations of 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L were used during the definitive test.

The 72-hour EC50 was >10,000 mg/L and the 72-hour NOEC 1,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Key value for chemical safety assessment

EC50 for freshwater algae:

10 g/L

EC10 or NOEC for freshwater algae:

1 000 mg/L