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EC number: 204-524-2 | CAS number: 122-14-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 September 2001 - 18 September 2001
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Principles of method if other than guideline:
- The effect of fenitrothion on the respiration of activated sewage sludge (from a water treatment works containing effluent of predominantly domestic origin) was examined according to OECD Guideline 209. Test solutions were prepared as follows:
Control solutions: two solutions of activated sludge at a concentration of 1.6 g/l in dechlorinated water.
Test solutions: five solutions of activated sludge as the control, but with the addition of fenitrothion at 10, 31.6, 100, 316 and 1000 mg/l
Reference solutions: three solutions of activated sludge as the control, but with the addition of the reference item, 3,5-dichlorophenol,at concentrations of 4, 12 and 36 mg/l.
The above solutions were incubated for three hours at 21°C, and then the oxygen consumption was monitored for a period of approximately 10 minutes using a chart recorder. The oxygen consumption of the test and reference item solutions were then compared with the controls and the EC50 (concentration causing 50% inhibition of the respiration rate) was then estimated. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Fenitrothion
- EC Number:
- 204-524-2
- EC Name:
- Fenitrothion
- Cas Number:
- 122-14-5
- Molecular formula:
- C9H12NO5PS
- IUPAC Name:
- O,O-dimethyl O-3-methyl-4-nitrophenyl phosphorothioate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- DO2 measured for 10 minutes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Each concentration was prepared separately by adding 16 mL of sewage feed to each of the flasks which were made up to 300 mL
with water only in the case of the controls or a nominal mixture of water and test item in the case of the test solutions.
Each mixture was then made up to 500 mL by adding 200 mL of inoculum. As 200 mL of inoculum were added in a final volume of 500 mL,
the organic material concentration from inoculum was 1.6 g/L (± 10%).
The solutions were aerated at between 0.5 to 1 liter of air per minute using a Pasteur-pipette connected with a flexible tube to an air pump as an aeration device.
Solutions were prepared approximately every 15 minutes for test times of 3 hours.
In order to verify the quality of the inoculum, the controls were prepared at the beginning (first control) and the end (second control) of the test.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- activated sludge collected from a water treatment works containing effluent from a predominantly domestic origin
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- Solutions were prepared approximately every 15 minutes for test times of 3 hours.
- Post exposure observation period:
- After 3 hours of aeration/contact time, the content of the test flask was poured into the measuring apparatus and the oxygen concentration was determined for a period of approximately 10 minutes.
Test conditions
- Hardness:
- 280 ± 20 mg/L
- Test temperature:
- 21°C
- pH:
- 6.0 - 8.0
- Dissolved oxygen:
- Initial DO conc TBC (supposedly saturated)
- Nominal and measured concentrations:
- Nominal: 10, 31.6, 100, 316 and 1000 mg/L
- Details on test conditions:
- 2.2.3 Environmental conditions during the test Temperature: 21°C.
Illumination: Dilution water:
Duration of test: Aeration:
no special precautions were taken.
dechlorinated tap water was used, with a hardness of 280 ± 20 mg/L as CaC03.
3 hours.
air was bubbled through each solution at the rate of 0.5 to 1 Umin
until the start of oxygen concentration measurements. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Respiration rates of the test and reference solutions.
The respiration rate of the test solution of highest concentration (1000 mg/L) was equivalent to the respiration rate of the first control (i.e. these rates were within 15% of each other).
Therefore, the oxygen consumption rate of the four other test solutions (10 to 316 mg/L) was not determined.
EC50s, based on nominal concentrations, were as follows:
Item 3-hour EC50 (mg/L) 95% confidence limits (mg/L)
Test item > 1000
Reference item16.0 16.0 13.8- 18.7
The difference between the two controls was <15% and the EC50 of the reference compound was between 5 and 30 mg/l (16 mg/l), therefore the test was
considered valid.
Any other information on results incl. tables
Effects on biological methods for sewage treatment - summary and conclusions
Type of study |
Test species & Time scale |
Dose range tested |
Result |
Reference |
Effects on biological methods for sewage treatment |
Activated sewage sludge (3 hours) |
10, 31.6, 100, 316 and 1000 mg/l |
EC50(3hour) : >1000 mg/l
|
L’Haridon J., 2002 (HW-0484) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- As the difference between the two controls was below 15% and the EC50 of 3,5-dichlorophenol was between 5 and 30 mg/L, the test was therefore considered valid.
- Conclusions:
- Due to the nature of use of the compound, i.e. agricultural and horticultural, sewage treatment plants are unlikely to be exposed to fenitrothion.
However, when the effect of the compound on the organisms involved in the treatment of wastewater was investigated, it had no effect on
the oxygen consumption of the sewage sludge inoculum at the highest dose tested with an EC50 of >1000 mg/l. Therefore fenitrothion has a
very low toxicity to the micro-organisms involved in the treatment of domestic waste water and will not adversely affect the sewage treatment processes. - Executive summary:
At the request of Sumitomo Chemical Agro Europe SA, Saint-Didier-au-Mont-d'Or, France, the effect of the test item, FENITROTHION, on the respiration of activated sewage sludge was evaluated using a 3-hour static test according to OECD guideline (No. 209, 4th April 1984).
Methods:
The concentrations of FENITROTHION tested were chosen according to EEC criteria for classification of dangerous substances towards aquatic organisms and also to determine suitable non-inhibitory concentrations to be used in biodegradability tests.
The EC50 (concentration expected to cause 50% inhibition of the respiration rate)
of the test item and the reference item was determined considering the oxygen consumption of the controls as 100%.
The validity criteria were met (the difference between the two controls was below 15% and the EC50 of 3,5-dichlorophenol was between 5 and 30 mg/L), the test was considered valid.
The respiration rate of the test solution of highest concentration (1000 mg/L) was equivalent to the respiration rate of the first control (i.e. these rates were within 15% of each other). Therefore, the oxygen consumption rate of the four other test solutions (10 to 316 mg/L) was not determined.
EC50s, based on nominal concentrations, were as follows:
3-hour EC50 (mg/L) 95% confidence limits (mg/L)
Test item: > 1000
Reference item: 16.0 13.8 - 18.7
The test item should be considered as non-toxic for the microorganisms of a the sewage treatment plant.
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