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EC number: - | CAS number: -
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: methods and results well documented
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- Environment Canada (2002)
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test substrate
- Vehicle:
- not specified
- Details on preparation and application of test substrate:
- Test compound added in solution to soil. Soil moisture was 35% (based on wet weight). Tests started after a 1 day equilibration period.
Test organisms
- Test organisms (species):
- Lumbricus sp.
- Animal group:
- annelids
Study design
- Study type:
- laboratory study
- Substrate type:
- other: artificial soil and natural soil
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20°C
- pH:
- artificial soil: 6.09
clay-loam reference soil: 6.05 - Moisture:
- 35%
- Details on test conditions:
- TEST SYSTEM
- No. of replicates per treatment group: 4
SOURCE AND PROPERTIES OF ARTIFICIAL SOIL
- Ingredients: 70% silica sand, 20% kaolinite clay, 10% spaghnum peat, 1-3g calcium carbonate per kg soil
- Soil texture
- % sand: 77.3
- % silt: 7.8
- % clay: 14.9
- Total carbon (%): 4.46
- Organic matter (%): 9
- pH water (1:2): 6.09
- eCEC: 18.5 cmol c/kg
- background B concentration: 3.5 mg B/kg dw
SOURCE AND PROPERTIES OF CLAY LOAM REFERENCE SOIL
- collected soil from Alberta (Canada)
- classified as a Delacour Orthic Black Chernozem.
- Soil texture
- % sand: 26.6
- % silt: 43.3
- % clay: 30.1
- Total carbon (%): 6.83
- Organic matter (%): 12.8
- pH water (1:2): 6.05
- eCEC: 34.5 cmol c/kg
- background B concentration: 1.0 mg B/kg dw
OTHER TEST CONDITIONS
- Photoperiod: 16h light, 8h dark - Nominal and measured concentrations:
- 0-500-1000-2000-4000-6000-8000 mg boric acid/kg dw,
corresponding to 0-87?5-175-350-700-1050-1400 mg B/kg dw. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 786.2 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- B
- Basis for effect:
- mortality
- Remarks on result:
- other: in artificial soil
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 501.4 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- B
- Basis for effect:
- mortality
- Remarks on result:
- other: in artificial soil
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 651.4 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- B
- Basis for effect:
- mortality
- Remarks on result:
- other: in clay loam reference soil
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 447.6 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- B
- Basis for effect:
- mortality
- Remarks on result:
- other: in clay loam reference soil
- Details on results:
- nominal added dosis; background concentration reported
- Reported statistics and error estimates:
- 7 and 14 day LCx values were generated using the Probit, Moving average and the trimmed Spearman-Karber methods.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- This study followed the OECD guideline. The standard test and selection criteria were fulfilled.
Test data was not presented.
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