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EC number: 442-390-9
CAS number: -
Table 1, Cell counts and growth rates in the algae test
Nominal conc. MV 31
(x 1E+04 cells/mL) at time:
Growth rate (µ) and reduction (%) at given intervals:
*Mean CV of growth rates. Section specific growth rate mean CV was 19%
The toxicity of MV 31 to the green algae, Pseudokirchneriella
subcapitata, was assessed in a 48-hour limit test conducted according to
the OECD 201 method. Test containers were sealed to avoid volatilization
losses of test material, and growth medium was amended with 100 mg/L
NaHCO3 to provide sufficient carbon dioxide and buffering to maintain
exponential growth. An initial limit test at 100 mg/L nominal
concentration indicated no statistically significant differences between
test and controls in section-by section growth rate, however the control
growth did did not meet validity criteria for the test method. A second
limit test was therefore completed. Exponential growth was observed
during the entire period of exposure in the control vessel and the
validity criteria were met in the second test. Results of the tests are
reported with respect to a nominal loading of 100 mg/L, as analytical
monitoring was not done. Test solutions were clear and colorless
throughout the test despite low water solubility of MV 31. Growth rates
at the end of the test were not significantly different between controls
and test samples. The EC50 is > 100 mg/L (nominal), which is far in
excess of the water solubility limit.
The study was performed in accordance with internationally-accepted test
guidelines and Good Laboratory Practice (GLP) standards. No analytical
monitoring was performed and the test substance, although loaded at a
rate well above its water solubility limit, was not visible in the test
solutions. Test chambers were sealed throughout the experiment to
prevent loss of material. Also, in the initial limit test, MV 31 was
visibly present in test chambers and section-by-section specific growth
rates were not significantly different. Therefore, this study is
reliable with restrictions and the results are suitable for purposes of
Risk Assessment, Classification & Labeling, and PBT Analysis.
Test Type: static (based on data obtained using OECD201 methodology)
48-hr ErC50: >100 mg/L (nominal concentration), 95% CI not calculable.
48-hr NOEC = 100 mg/L (nominal concentration, growth rate).
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