(ethyl-3-oxobutanoato-O'1,O'3)(2-dimethylaminoethanolato)(1-methoxypropan-2-olato)aluminium(III), dimerised

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1984

Deviations:

yes

Remarks:

Because of the limited stability of the test substance the exposure period was 24 hours instead of 48 hours. This is in accordance with the EEC guidelines mentioned in the protocol

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(ethyl-3-oxobutanoato-O'1,O'3)(2-dimethylaminoethanolato)(1-methoxypropan-2-olato)aluminium(III), dimerised

EC Number:

402-370-2

EC Name:

(ethyl-3-oxobutanoato-O'1,O'3)(2-dimethylaminoethanolato)(1-methoxypropan-2-olato)aluminium(III), dimerised

Cas Number:

149057-70-5

Molecular formula:

C14H28AlNO6

IUPAC Name:

octaaluminium(3+) octakis((2Z)-4-ethoxy-4-oxobut-2-en-2-olate) octakis(1-methoxypropan-2-olate) octakis(2-(dimethylamino)ethan-1-olate)

Test material form:

liquid

Details on test material:

purity not specified, batch 25/2/87R

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The samples taken from the test media (approx. 100 ml) were treated as follows:
10 ml of a sample was led through an activated Sep C18 column followed by eluation with 1 ml methanol (Merck, lichrosolv), The 100 µl eluent was diluted with methanol and internal standard in such a way that the response of the gas chromatograph was comparable with that recorded for a calibration standard of concentrations approximately of 250 mg/L.

Test solutions

Vehicle:

yes

Details on test solutions:

Dutch Standard Water (DSW)

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

not specified

Water media type:

other: Dutch Standard Water (DSW)

Total exposure duration:

24 h

Test conditions

Hardness:

11.7 DH

pH:

8.2 +/- 0.2

Nominal and measured concentrations:

nominal: 320, 560, 1000 mg/L
actual after 0h: 100, 145, 230 mg/L

Reference substance (positive control):

yes

Remarks:

K2Cr207 {Merck-Darmstadt, Germany) was used as a reference substance at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/1.

Results and discussion

Effect concentrations

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Conclusions:

The present study reveals that A6/1/0 is toxic to Daphnia magna at nominal concentrations higher than 320 mg/1 in a 24h immobility test according to EEC method C2.

Executive summary:

The present study reveals that A6/1/0 is toxic to Daphnia magna at nominal concentrations higher than 320 mg/L in a 24h immobility test according to EEC method C2.