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EC number: 204-729-7 | CAS number: 125-20-2
The water solubility of the test item has been determined in a GLP study according to OECD 105 and EU method A.6.
In a preliminary study, the solubility of the test item in water was estimated as < 10 mg/L.
The column elution method was used for the determination of the solubility of the test item in water. Quartz sand was coated with test item and filled into a stainless steel column (150*4.6 mm). A flow rate of 25.01 mL/h was set. After discarding of the first fraction (run time 180 min), fractions were collected and measured. After seven consecutive fractions, the plateau was considered as reached, because variation was less than 30 % and no upward tendency was observed. A second run with a flow rate of 12.52 mL/h and a third run with a flow rate of 6.03 mL/h was performed likewise. No differences above 30% in the concentrations of the solutions could be determined between the sample dates of the different flow rates, supporting the independency of solubility and flow rate.
At the plateau, the concentration of 3,3-bis(4-hydroxy-5-isopropyl-o-tolyl)phthalide in water had reached: 2.59 ± 0.50 µg/L at 20 ± 0.5 °C (mean value of 15 fractions)
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