Xanthylium, 9-(2-carboxyphenyl)-3,6-bis(diethylamino)-, molybdatesilicate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2 March to 14 May 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Source Sposor,batch no LF 951020
- Expiration date of the lot/batch: not stated
- Purity test date: not tated


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: roomtemperature in the dark
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method:
- Sample storage conditions before analysis:

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test material was prepared as WAF (water accomodated fraction) in dechlorinated tap water.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Juvenile Rainbow Trout
- Strain: Oncorhynchus mykiss
- Source: Commercial supplier
- Age at study initiation (mean and range, SD): not stated
- Length at study initiation (length definition, mean, range and SD): 4.5cm (sd= 0.2cm)
- Weight at study initiation (mean and range, SD): 1.14g (sd = 0.12g)
- Method of breeding: n/a
- Maintenance of the brood fish: n/a

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food during acclimation: commercial trout pellets
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): 2% mortality in the 7 days prior to the start of the test

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

FEEDING DURING TEST
- Food type:
- Amount:
- Frequency:

Test type:

semi-static

Water media type:

other: dechlorinated tap water

Limit test:

no

Total exposure duration:

96 h

Hardness:

14.0 - 14.5

Test temperature:

13.5 - 14.5 C

pH:

7.6 - 7.9

Dissolved oxygen:

9.1 - 9.9 mg O2/L

Nominal and measured concentrations:

Nominal concentrations 10, 18, 32, 56 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 litre glass exposure vessals
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume:
- Aeration: yes
- Type of flow-through (e.g. peristaltic or proportional diluter): no
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF's prepared

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96 -hour LLR50 (Lethal loading rate) value of 40 mg/L loading rate WSF with 95% confidece limits of 36 - 45 mg/L loading rats WSF. The No Observed Effect Loading Rate was 18 mg/L loading rate WSF.

Executive summary:

The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96 -hour LLR50 (Lethal loading rate) value of 40 mg/L loading rate WSF with 95% confidece limits of 36 - 45 mg/L loading rats WSF. The No Observed Effect Loading Rate was 18 mg/L loading rate WSF.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

18 mg/L

Additional information

The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96 -hour LLR50 (Lethal loading rate) value of 40 mg/L loading rate WSF with 95% confidence limits of 36 - 45 mg/L loading rats WSF. The No Observed Effect Loading Rate was 18 mg/L loading rate WSF.