Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes
Remarks:
ICP/OES

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Freshwater unicellular green alga Pseudokirchneriella subcapitata of the chlorococcal order strain number CCAP 278.4 was used in this study.

Study design

Test type:
not specified
Water media type:
other: ultrapure water
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
not specified
Test temperature:
22.7 °C – 23.3°C
pH:
7.5 (t=0) to 9.4 (t=72h)
Dissolved oxygen:
not specified
Nominal and measured concentrations:
10, 32, 102, 328, 1050 mg/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
ca. 139.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
ca. 141.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The results obtained for the test item “ W3803S04SOLSIV” on the toxicological endpoint “inhibition of growth rate” indicate an ErL50-(0-72h) = 139.9 mg/L [99.4 – 200.3], based on nominal test item loading rate.
The results obtained for the test item “ W3803S04SOLSIV” on the toxicological endpoint “Yield inhibition” indicate an EyL50-(0-72h) = 141.7 mg/L [93.6 – 222.7], based on nominal test item loading rate.