α,α,α-trifluoro-3-tolualdehyde

Long-term toxicity to fish

Administrative data

Endpoint:

fish early-life stage toxicity

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1400 (Fish Early-life Stage Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α-trifluoro-3-tolualdehyde

EC Number:

207-228-1

EC Name:

α,α,α-trifluoro-3-tolualdehyde

Cas Number:

454-89-7

Molecular formula:

C8H5F3O

IUPAC Name:

3-(trifluoromethyl)benzaldehyde

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Stock Solution Preparation: approx. 125.0 µg/L diluted

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

Fathead minnow embryos that were < 24 hr old were obtained from the University of Wisconsin-Superior culture unit, and all embryo masses were pooled. Approximately 42-47 embryos were randomly selected and transferred to the exposure chamber (84-94 fry/chamber). At hatch, 15 fry from each egg cup and its replicate were randomly selected and transferred to each exposure chamber.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

33 d

Test conditions

Hardness:

51.73 CaC03 (mg/L)

Test temperature:

25.58 °C (mean value)

pH:

7.22 (mean value)

Dissolved oxygen:

6.90 mg/L (mean value)

Salinity:

n.a.

Conductivity:

not recorded

Nominal and measured concentrations:

Nominal test concentration (µg/L): 52, 104, 208, 417 and 833
equal
Measured uncorrected concentration (µg/L) (mean value out of two determinations): 53.6, 57.8, 66.6, 116.5 and 285.3

Details on test conditions:

The test was performed with a modified Benoit flow-through mini-diluter.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

285 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

number hatched

Effect concentrations 2

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

117 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

117 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

weight

Remarks:

wet

Effect concentrations 4

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

117 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

weight

Remarks:

dry

Effect concentrations 5

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

117 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

length

Remarks:

standard

Effect concentrations 6

Duration:

33 d

Dose descriptor:

LOEC

Effect conc.:

285 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: Percent survival; wet weight; dry weight; length standard

Details on results:

- Average Spike Recovery (%):100.00 n= 0
- Duplicate Analysis Agreement(%): 83.8 n= 11

Reported statistics and error estimates:

Percent Normal Larvae at Hatch and Percent Survival data were transformed using the Freeman-Tukey Arc sine method prior to analysis by William's TeSt. The
Wet Weight and Length data were analyzed using William's Test (P<0.05), while the Dry Weight data were analyzed using Dunnett's Test.

Any other information on results incl. tables

Juvenile growth at 33 days:

- wet weight (mg): 70.1 -146.0

- dry weight (mg): 13.1 -30.4

- length (mm): 14.7 -19.6

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In the test with 3-(Trifluoromethyl)benzaldehyde (CAS no. 454-89-7) with fish (Pimephales promelas) a NOEC of 117 µg/L and a LOEC of 285 µg/L after 33 dyas are obtained concerning the percent survival, wet weight, dry weight and length standard of the juvenile growth. The test guideline is equivalent to EPA OPPTS 850.1400 (Fish Early-life Stage Toxicity Test).

Executive summary:

In the test with 3-(Trifluoromethyl)benzaldehyde (CAS no. 454-89-7) with fish (Pimephales promelas) a NOEC of 117 µg/L and a LOEC of 285 µg/L after 33 dyas are obtained concerning the percent survival, wet weight, dry weight and length standard of the juvenile growth. The test guideline is equivalent to EPA OPPTS 850.1400 (Fish Early-life Stage Toxicity Test).