5-hydroxyiminobarbituric acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015.03.09-2015.03.11

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

No verification of test concentrations

Principles of method if other than guideline:

20 daphnids devided in 4 groups were exposed to the test item for 48 hours. Immobility was monitored every 24 hours and an EC50 and a NOEC value determined.
As a negative control standard freshwater (ISO6341) was applied.
Daphnids were not feed during the study

GLP compliance:

no

Remarks:

The kit from Microbiotests has been extensivley used at our laboratory and database at our laboratory shows that results obtained with the kit are reliable and valid to be used for assesment of acute toxicity towards daphnids.

Test material

Test material information

Constituent 1

Reference substance name:

5-hydroxyiminobarbituric acid

EC Number:

201-741-4

EC Name:

5-hydroxyiminobarbituric acid

Cas Number:

87-39-8

Molecular formula:

C4H3N3O4

IUPAC Name:

5-(hydroxyimino)-1,3-diazinane-2,4,6-trione

Test material form:

solid: particulate/powder

Details on test material:

Lot/batch No.: 201713807

Specific details on test material used for the study:

Batch number: BFB083-172

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

no sampling

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test organisms were recieved from Ghent University in Belgium, Laboratory for Biological Research in Aquatic Pollution. DAPHTOXKIT FTM Magna from Microbiotests, containing Ephippia. Daphnids were < 24 h old at the initiation of the test.

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

not included

Test conditions

Hardness:

NA

Test temperature:

20-22 degrees celcius

pH:

6.95

Dissolved oxygen:

Test media was saturated with oxygen before the test was initiated

Salinity:

NA

Conductivity:

NA

Nominal and measured concentrations:

Nominal: 6.25, 12.5; 25; 50; 100 mg/L

Reference substance (positive control):

no

Remarks:

every 6 month a study with potassiumdichromate is conducted

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

6.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

10.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

6.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

10.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Endpoint exammined: Immobility

Reported statistics and error estimates:

Software applied for calculation of the EC50 and NOEC value: ToxRAT

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A NOEC and an EC50 of 6.3 mg/L and 10.8 mg/L determined after 24 hours.
After 48 hours the corresponding values were 6.3 mg/L and 10.6 mg/L.

Executive summary:

The acute toxicity of the test item Violuric acid towards invertebrate (Daphnia magna) was tested according to the principles described in the OECD Guideline no. 202.

20 daphnids/concentration devided in 4 groups were exposed for 48 hours and immobility assessed after 24 and 48 hours. Daphnids were not feed during the study and no analytical verifications of the test concentrations were performed.

A NOEC and an EC50 of 6.3 mg/L and 10.8 mg/L determined after 24 hours. After 48 hours the corresponding values were 6.3 mg/L and 10.6 mg/L.