4-(3-formyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy)benzonitrile

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 Mar 2018 to 20 Apr 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Version / remarks:

OECD Guideline 209. "Activated Sludge, Respiration Inhibition Test (Carbon and
Ammonium Oxidation)”, adopted 22 July 2010.

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-[3-formyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy]benzonitrile

EC Number:

948-544-6

Cas Number:

2141947-89-7

Molecular formula:

C20H20BNO4

IUPAC Name:

4-[3-formyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy]benzonitrile

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Purity/Composition: 99.73%

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Details on test solutions:

First full test: Six concentrations: 10, 22, 56, 100, 220 and 560 mg/L (See deviation in Appendix 7).
Second full test: Seven concentrations: 4.8, 10, 22, 46, 100, 220 and 480 mg/L.

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Study design

Test type:

not specified

Water media type:

freshwater

Remarks:

Tap water purified by reverse osmosis (Millipore Corp., Bedford, Mass., USA).

Limit test:

yes

Total exposure duration:

3 h

Test conditions

Test temperature:

First Full Test: 20 ± 2°C.
Second Full Test: between 17.4 and 21.0°C.

pH:

7.5 ± 0.5.

Dissolved oxygen:

dissolved oxygen concentration at the start was above 60-70% saturation

Nominal and measured concentrations:

First full test: Six concentrations: 10, 22, 56, 100, 220 and 560 mg/L (See deviation in Appendix 7).
Second full test: Seven concentrations: 4.8, 10, 22, 46, 100, 220 and 480 mg/L.

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

4.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

ca. 32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Effect concentrations 3

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

ca. 232 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

EC20 64 mg/L

Results with reference substance (positive control):

The batch of activated sludge was tested for sensitivity with the reference item 3,5-dichlorophenol, and showed normal sensitivity.

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Conclusions:

In conclusion, under the conditions of this present test PF-06961030 was not toxic to waste water bacteria (activated sludge) at or below a concentration of 4.8 mg/L (NOEC).
The EC10 was 32 mg/L (95% confidence interval: 26 - 39 mg/L).
The EC20 was 64 mg/L (95% confidence interval: 54 - 73 mg/L).
The EC50 was 232 mg/L (95% confidence interval: 210 - 258 mg/L).