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EC number: 218-746-2 | CAS number: 2224-15-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Source: National Institute of Environmental Research (42, Hwangyeong-ro, Seo-gu, Incheon, Republic of Korea)
- Length at study initiation: range-finding study: 2.26–2.58 cm; main study: 2.10–2.47 cm
- Weight at study initiation: range-finding study: 0.218–0.268 g; main study: 0.219–0.266 g
- Method of breeding: Upon receipt, suitable male and female fish, at a ratio of 3 to 2 without any visible abnormalities and of similar size, were selected and placed in 50 L of breeding water. Approximately 25 fish (±10%) were bred. Eggs were harvested in breeding chambers and placed in hatching chambers. After hatching, the fry were placed in breeding chambers maintained at 23±1°C and bred. Approximately 30% of holding water was replaced once a week. The holding room was artificially illuminated; a 16-hour artificial light and 8-hour dark cycle. Fish were fed Brine shrimp (Ocean Star International, Inc., U.S.A.) in the morning and Top Meal (Jaeilfeed Co., Republic of Korea), at an amount corresponding to approximately 3% of their body weight, in the afternoon daily except from Saturday afternoon to Sunday, when they were fasted.
ACCLIMATION
- Acclimation period: 10 days
- Acclimation conditions: same as test
- Type and amount of food during acclimation: Brine shrimp (Ocean Star International, Inc., U.S.A.) in the morning and Top meal (Jaeilfeed Co., Republic of Korea) in the afternoon, at an amount of approximately 3% of their body weight, once daily except from Saturday afternoon to Sunday when they were fasted.
- Health during acclimation (any mortality observed): During 7 days prior to exposure, the mortality of a batch of fish was < 5% of the population.
FEEDING DURING TEST
- Frequency: Fish were not fed from 24 hours prior to exposure until the end of exposure. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- range-finding study: 22.3 - 22.8 °C
main study: 22.2 - 22.8 °C - pH:
- range-finding study: 7.58 - 7.74
main study: 7.66 - 7.80 - Dissolved oxygen:
- range-finding study: 8.54 - 8.92
main study: 8.14 - 8.45 - Nominal and measured concentrations:
- Range-finding study
Nominal: 0, 0.1, 1, 10, 20 and 100 mg/l (nominal)
Main study
Nominal: 0, 30.1, 40.6, 54.9, 74.1 and 100 mg/l
Measured (corrected for recovery) at 0 h: N.D, 31.51, 42.39, 57.95, 75.09, 97.71 mg/l
Measured (corrected for recovery) at 96 h: N.D, 32.49, 41.27, 52.80, 70.90, 95.36 mg/l - Reference substance (positive control):
- yes
- Remarks:
- but not concurrent
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 63.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
Oryzias latipes were exposed once every 6 months to the reference substance (Copper (II) Sulfate) for 96 h. The recent result (LC50) of the positive control study was 0.74 mg/L, which was within the permissible range of historical data (mean±2SD: 0.26–0.70 mg/L) of Biotoxtech Co., Ltd. Exposure conditions of the positive control study were identical to the conditions of the definitive study. - Validity criteria fulfilled:
- yes
- Remarks:
- The mortality in the control group was 0%. The dissolved oxygen concentrations in the treatment groups and control group were maintained at more than 60% of the air-saturation values.
- Executive summary:
This study was designed to assess the acute toxicity and to determine the median lethal concentration (LC50) of the test substance, 2,2’-[ethylenebis(oxymethylene)]bisoxirane, in Ricefish (Oryzias latipes) during an exposure period of 96 hours under static test conditions. Based on the result of the range finding study, the definitive study was conducted and the treatment levels were selected at 30.1, 40.6, 54.9, 74.1 and 100 mg/L, and a control group was prepared for this study.
During the exposure period, the mortality in the control group was 0%. The dissolved oxygen concentrations in the treatment groups and control group were maintained at more than 60% of the air-saturation values. Therefore, the results were considered to be acceptable.
As a result of the concentration analysis of the test substance, the measured values in the 30.1, 40.6, 54.9, 74.1 and 100 mg/L treatment groups during the exposure period were from 80 to 120% when compared to the nominal concentrations. Therefore, all test results were determined based on the nominal concentrations.
Based on the result of this study, the LC50 values obtained under the test conditions with the test substance, 2,2’-[ethylenebis(oxymethylene)]bisoxirane, were determined to be >100 mg/L at 24 and 48 h after exposure, 73.4 mg/L (95% confidence limits: 64.6–83.6 mg/L) at 72 h after exposure and 63.8 mg/L (95% confidence limits: 56.3–72.2 mg/L) (measured concentration) at 96 h after exposure.
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 63.8 mg/L
Additional information
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