Amides, C16-18 (even numbered) saturated and C18 unsaturated, N,N'-[[(2-hydroxyethyl)imino]di-2,1-ethanediyl]bis-, ethoxylated (1-2.5 mol)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Eg 203


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 4°C

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

Stock solution of 1000 mg/L in dechlorinated tap water; sonification for 15 min. Dilution to actual test concentrations with dechlorinated tap water; sonification for 15 min.
In the preliminary test, test solutions were prepared only once prior to initiation of the test. In the main test, the preparation of fresh test solutions was on a daily basis.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain:
- Source: Aquafarm RYBA GmbH, D-2730 Zeven

ACCLIMATION
- Acclimation period: minimum of 14 days
- Acclimation conditions (same as test or not): same

FEEDING DURING TEST
- Food type: Tetra min
- Frequency: once daily

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

14°dH

Test temperature:

25°C +- 1.5°C

pH:

8.0 +- 0.5

Dissolved oxygen:

> 60% of air saturation (ca. 6.0 mgO2/L)

Nominal and measured concentrations:

nominal 0.001, 0.01, 0.1, 1.0 and 10 g/L (preliminary test)
nominal:1.9, 2.6, 3.6, 5.1, 7.1, 10.0, 14.0, 19.0, 26.0 and 36.0 g/L (main test)

Details on test conditions:

TEST SYSTEM
- Test vessel: 12 L glass containers
- Aeration: continuous aeration using a membrane pump system
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel:10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified tap water

OTHER TEST CONDITIONS
- Adjustment of pH: -
- Photoperiod: light for 16h daily
- Light intensity:600 - 800 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.4
- Justification for using less concentrations than requested by guideline:
- Range finding study: yes
- Test concentrations: 0.001, 0.01, 0.1, 1, 10 g/L
- Results used to determine the conditions for the definitive study: yes

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.9 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

LC50 (96h) = 3 g/L.

Description of key information

LC50 (96h) = 3 g/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

3 g/L