Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
23 Oct - 24 Nov 2014
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Aerobic sludge originating from the mixed treatment plant of urban (66%) and industrial (34%) liquid sewage situated at San Rocco-Monza (MI).
- Preparation of inoculum for exposure: in the laboratory, the sampled sludge was mixed and let to settle in aerobic conditions. Before use, the sludge was cenfrifuged, washed and alaysed for the concentration of suspended solids.
The concentration of suspended solids was about 4 mg/L.
Duration of test (contact time):
28 d
Initial conc.:
20.4 mg/L
Based on:
TOC
Parameter followed for biodegradation estimation:
inorg. C analysis
Details on study design:
TEST CONDITIONS
- Composition of medium:
Solution A:
KH2PO4: 8.5 g/L
K2HPO4: 21.75 g/L
Na2HPO4 x 2H2O: 33.4 g/L
NH4Cl: 0.5 g/L

Solution B:
CaCl2 x 2H2O: 36.4 g/L

Solution C:
MgSO4 x 7H2O: 22.5 g/L

Solution D:
FeCl3 x 6H2O: 0.25 g/L

10 mL of Solution A was mixed with 800 mL of deionised water. 1 mL of Solution B, C and D was added. The deionised water was finally added up to 1000 mL volume.

- Test temperature: 25°C ± 2°C
- pH adjusted: no
- Aeration of dilution water: no
- Suspended solids concentration: 4 mg/L
- Continuous darkness: yes


TEST SYSTEM
- Culturing apparatus: glass bottles with hermetic seal
- Number of culture flasks/concentration: 5
- Measuring equipment: automatic TOC analyser (SHIMADZU).

SAMPLING
- Sampling frequency: weekly

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 5 bottles
- Abiotic sterile control: no
- Toxicity control: yes, 5 bottles
Reference substance:
benzoic acid, sodium salt
Remarks:
concentration 20.1 mg/L (TOC)
Key result
Parameter:
% degradation (inorg. C analysis)
Value:
91
Sampling time:
28 d
Results with reference substance:
biodegradation of 114% after 28 days

Biodegradation of the reference substance and test substance:

Day % biodegradation
reference substance
% biodegradation
test substance
0 0 0
1 49 9
7 100 66
14 100 77
21 109 82
28 114 91
Validity criteria fulfilled:
yes
Interpretation of results:
other: biodegradable
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
14 Jul - 11 Aug 2009
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
no
Remarks:
Test was a inner test performed in our lab as screening. We are not an external lab GLP certified
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: clarification plant Niederlahnstein
- Storage conditions: not stated
- Preparation of inoculum for exposure: not stated
- Concentration of sludge: 29.7 mg/L
Duration of test (contact time):
28 d
Initial conc.:
108.6 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
radiochem. meas.
Details on study design:
TEST CONDITIONS
- Composition of medium: not stated
- Test temperature: not stated

TEST SYSTEM
- Culturing apparatus: not stated
- Number of culture flasks/concentration: not stated


SAMPLING
- Sampling frequency: daily
Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (O2 consumption)
Value:
89
Sampling time:
28 d
Results with reference substance:
97% after 28 days

Biodegradation results:

Day Biodegradation %
Test substance Reference substance
1 25 19
2 47 41
3 56 58
4 55 66
5 62 70
6 65 74
7 67 77
8 69 80
9 71 82
10 72 84
11 73 85
12 75 87
13 76 87
14 78 88
15 80 89
16 80 89
17 81 90
18 81 90
19 81 91
20 82 91
21 84 93
22 82 93
23 84 94
24 88 95
25 87 96
26 89 96
27 89 96
28 89 97
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
15 Dec 1995 -12 Jan 1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes (incl. QA statement)
Remarks:
1996, 27th February, The Department of Health of the Government of the United Kingdom
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Sewage treatment plant Hochdahl (1mL/L culture solution)
Duration of test (contact time):
28 d
Initial conc.:
2 mg/L
Based on:
act. ingr.
Initial conc.:
5 mg/L
Based on:
act. ingr.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 20 ± 1 °C
- Continuous darkness: yes

SAMPLING
- Sampling frequency: Day 0, day 7, day 21 and day 28



Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (O2 consumption)
Value:
> 90 - < 100
Sampling time:
28 d
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Justification for type of information:
Refer to the Analogue Approach Justification document provided in IUCLID Section 13.
Reason / purpose for cross-reference:
read-across source
Parameter:
% degradation (O2 consumption)
Value:
> 90 - < 100
Sampling time:
28 d
Remarks on result:
other: CAS 85586-07-8

Description of key information

Readily biodegradable(WoE approach)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Ready biodegradation of C12-14 AS Zn is assessed based on weight of evidence approach. Two reliable studies investigating ready biodegradation of C12-14 AS Zn are available. The first GLP study (Z&S, 2014) was performed according to OECD 301B guideline. Activated sludge originated from a mixed treatment plant of urban and industrial liquid sewage. The test was carried out with the concentration of the test substance 20 mg/L TOC. The biodegradation reached 91% after 28 days and therefore the substance can be considered as biodegradable. The second non-GLP study (Z&S, 2009) was conducted according to OECD 301F guideline. Activated sludge from sewage treatment plant was used as inoculum. Oxygen consumption was followed by 28 days. At the end of the test biodegradation rate of 89% was reached. The test item is considered to be readily biodegradable.

Moreover, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5, a read-across to a structurally related substance C12-14 AS Na (CAS 85586-07-8) was conducted. A GLP study was performed according to OECD guideline 301 D (BASF, 1996). Activated, non-adapted domestic sludge was used as inoculum. The biodegradation after 28 days reached >90% (as O2 consumption) and the substance was considered as readily biodegradable.

In general, ready biodegradability tests are available for several substances belonging to the category of alkyl sulfates. Based on the results of screening tests it can be concluded that alkyl sulfates with carbon chain length of C8-C20 are readily biodegradable according to OECD criteria (OECD, 2007; HERA, 2002).

Based on the available evidence, it can be concluded that C12-14 AS Zn is readily biodegradable according to OECD criteria.