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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1972
Report Date:
1970

Materials and methods

Principles of method if other than guideline:
according to internal standard method
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
other: Kislegg
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: between 24 and 43 g

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: aequous emulsion with Traganth
Details on exposure:
- Concentration in vehicle: 2% and 4%
Doses:
200, 250, 320, 400 mg/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 253 mg/kg bw
Mortality:
All animals treated with 400 mg/kg died. At 320 mg/kg 4/5 males and 3/5 females died, at 250 mg/kg, 3/5 males and 3% females died. At 200 mg/kg one male and one female died.
Clinical signs:
Immediately after administration accelerated breathing, caved in sides, unsteady gait and flattened ears. After 3.5 hours animals appeared sedated, irregular breathing, closed eye lids, ruffled fur. During the observation period animals showed ruffeld fur, rapid breathing and curved pody position and bad general health. Recovery from these symptoms after 10 to 11 days.
Gross pathology:
Occationally substance incorporation into the spleen and liver, adhesions in the liver

Applicant's summary and conclusion