1,3,5-Triazine-2,4,6-triamine, deammoniated

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-05-25 - 1994-06-30

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The GLP study was conducted according to an internationally accepted guideline (OECD 405). All study parameters are based on the specific guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,3,5-Triazine-2,4,6-triamine, deammoniated

EC Number:

272-034-6

EC Name:

1,3,5-Triazine-2,4,6-triamine, deammoniated

Cas Number:

68649-66-1

Molecular formula:

see structural formula below

IUPAC Name:

1,3,5-Triazine-2,4,6-triamine, deammoniated

Test animals / tissue source

Species:

rabbit

Strain:

other: White russian (Albino)

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

44.7 - 49.3 mg (representing a volume of about 0.1 ml)

Duration of treatment / exposure:

72 hours

Observation period (in vivo):

72 hours

Number of animals or in vitro replicates:

3 males

Details on study design:

After grinding in a ball mill 44.7 - 49.3 mg (representing a volume of about 0.1 ml) of the powdered test substance were applied into the conjunctival sac of the left or right eye. Then both lids were briefly closed by gentle finger pressure. The other eye of each animal was not treated and acted as control. The treated eye was not rinsed. The treated eye was examined using a Cliptrix pencil light (supplied by Varta, D-30419 Hannover, No. 645). Signs of irritation on the cornea, iris, and conjunctiva were assessed quantitatively and qualitatively using the DRAIZE scale (1, 2, 4, 5). In addition, attention was paid to the occurrence of further reactions on the eye (e.g. corrosive effects) and to clinical symptoms (systemic toxic effects).

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 72 hours

Score:

2

Max. score:

110

Reversibility:

fully reversible

Results 2

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

not specified

Results 3

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

2

Reversibility:

not specified

Results 4

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible

Results 5

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

not specified

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Durferrit REG 1 was tested for eye irritating properties in the rabbit eye.
Observed eye lesions included hyperemia, diffuse redness, slight discharge or discharge with moistening of lids and hair. The symptoms were fully reversible after 48 hours. It is concluded that REG 1 is non-irritant to the eyes of the rabbit.

Executive summary:

Durferrit REG 1 was tested for eye irritating properties in the rabbit eye. Observed eye lesions included hyperemia, diffuse redness, slight discharge or discharge with moistening of lids and hair. The symptoms were fully reversible after 48 hours. It is concluded that REG 1 is non-irritant to the eyes of the rabbit.