Terpene hydrocarbon alcohols, terpene processing by-products

Short-term toxicity to fish

Description of key information

The Terpene hydrocarbon alcohols' fish toxicity is based on read-across from the close structural analogue Terpinolene.

Terpinolene and its acute fish toxicity

An acute toxicity study was performed according to OECD TG 203 and in compliance with GLP criteria. Following a preliminary range-finding test, groups of seven zebrafish (D. rerio) were exposed to Terpinolene at nominal test concentrations of 0.6, 0.8, 1.1, 1.5 and 2.0 mg/L, in aquaria closed over the study period with a minimum of headspace. The total test period was 96 hours and test solutions were renewed on a daily basis. The mortality of the fish was determined in a semi-static 96-h test by visual observation after 24, 48, 72 and 96 h. Exposure concentrations were measured at the start of the test and every 24 h thereafter in old and new solutions. Concentrations of the test item were not satisfactorily maintained within ± 20 % of the initial concentrations throughout the test. Thus, the evaluation of the effects on fish was based on the geometric mean of the measured concentrations: 0.48, 0.65, 0.93, 1.12 and 1.48 mg/L. Mortalities were recorded at 3, 6, 24, 48, 72 and 96 h. Sub-lethal effects of lack of balance /swimming on the back were observed in the groups exposed to 0.93, 1.12 and 1.48 mg/L of test substance. Based on mean measured concentrations, the 96-h LC50 of Terpinolene to zebrafish was 0.805 mg/L.  

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.805 mg/L