Pyrogallol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: ANFOR T90-303 Juin 1978

Version / remarks:

Essais des eaux. Détermination de la toxicité aigüe d'une substance vis à vis de Brachydanio Rerio (essai statique).

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

Deviations:

no

GLP compliance:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: not specified
- Expiration date of the lot/batch: not specified
- Purity test date: not specified

RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: not applicable
- Specific activity: not applicable
- Locations of the label: not applicable
- Expiration date of radiochemical substance: not applicable

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability under test conditions: not specified
- Solubility and stability of the test substance in the solvent/vehicle: not specified
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not specified

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: according to the reported guideline
- Preliminary purification step (if any): -
- Final dilution of a dissolved solid, stock liquid or gel: -
- Final preparation of a solid: -

FORM AS APPLIED IN THE TEST (if different from that of starting material) -

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable) -

OTHER SPECIFICS: -

Analytical monitoring:

not specified

Details on sampling:

according to the reported guideline

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain: Hamilton Buchanan
- Source: not specified
- Age at study initiation (mean and range, SD): not specified
- Length at study initiation (length definition, mean, range and SD): 2.5 ± 0.2 cm (without the tail)
- Weight at study initiation (mean and range, SD): not specified
- Method of breeding: not specified
- Maintenance of the brood fish: Fishes are bought at maturity and then raised in the laboratory in an aquarium with a capacity of 100 liters containing 70 liters of "aged" water.

ACCLIMATION
- Acclimation period: The acclimation period lasts as a minimum two weeks before use for the tests
- Acclimation conditions (same as test or not): not specified
- Type and amount of food during acclimation: not specified
- Feeding frequency during acclimation: not specified
- Health during acclimation (any mortality observed): not specified

QUARANTINE (wild caught)
- Duration: not specified
- Health/mortality: not specified

FEEDING DURING TEST
- Food type: TETRAMIN (commercial food).
- Amount: not specified
- Frequency: the fish are fed daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Remarks on exposure duration:

At different reading times, mortalities are recorded.

Hardness:

150 mg/L CaCO3

Test temperature:

22 deg C

pH:

pH 7.8-8

Dissolved oxygen:

no data

Salinity:

no data

Conductivity:

no data

Nominal and measured concentrations:

Concentration:
35000-100000 ug/L for 2 and 4 hours
10000-35000 ug/L for 6 and 24 hours

Details on test conditions:

TEST SYSTEM
- Test vessel: The aquariums are covered with a glass plate during the tests
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: aquariums with a capacity of 7 liters
- Aeration: no aeration is practiced
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate): not specified
- No. of organisms per vessel: Five fishes were used for each concentration.
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified
- No. of vessels per vehicle control (replicates): not specified
- Biomass loading rate: not specified

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Fish toxicity tests are carried out in aquariums with a capacity of 7 liters containing 2 liters of test solution. - Total organic carbon: not specified
- Particulate matter: not specified
- Metals: not specified
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: not specified
- Ca/mg ratio: not specified
- Culture medium different from test medium: not specified
- Intervals of water quality measurement: not specified

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified
- Light intensity: the fihes were placed in controlled room under a natural lighting.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not specified
- Justification for using less concentrations than requested by guideline: not specified
- Range finding study not specified
- Test concentrations: < 1 mg/I, 1-3,5; 3,5-10; 10-35; 35-100 and >= 100 mg/l
- Results used to determine the conditions for the definitive study: AFNOR T 90-303 (1978)

Reference substance (positive control):

yes

Duration:

2 h

Dose descriptor:

LC50

Effect conc.:

> 35 000 - < 100 000 µg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

4 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 - < 35 000 µg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

6 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 - < 35 000 µg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Key result

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 - < 35 000 µg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The LC 50 (24h) was in the range 10-35 mg/l  for Brachydanio reri, at test described test conditions.
Considering the results, accoring to CLP classification critiria, the substance was judged as hazardous to the aquatic environment: the substance has an harmonized classification as Aquatic Chronic 3 H412

Executive summary:

The acute toxicity of analytical grade of Pyrogallol has been tested on Brachydanio rerio after different periods of contact: 2, 4, 6 and 24 hours. The LC50 were determined.

Description of key information

The acute toxicity of Pyrogallol was tested on Brachydanio rerio and observed at 2, 4, 6 and 24 hours, according to ISO/DIS 7346/1; the preliminary test was performed according to AFNOR T 90-303 (1978).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

10 mg/L

Additional information

The LC 50 (24h) was in the range 10-35 mg/l  for Brachydanio rerio, at test described test conditions.

According to the results, the substance was considered as hazardous for aquatic environment and classified as Aquatic chronic 3 H412.