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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Please see Analogue Approach
Reason / purpose:
read-across source
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
other: loading rate (Water Accommodated Fraction )
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
loading rate (water accomodated fraction)
Basis for effect:
mobility
Conclusions:
At the saturated solution with a nominal loading of 100 mg/L of the test item 1,2,3-Propanetricarboxylic acid, 2-Hydroxy-, tris(C12-C13-branched-alkyl)ester, no effects were observed on Daphnia magna. Thus, the test item is not toxic in the range of the water solubility.
Executive summary:

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of a saturated solution with a nominal loading of 100 mg/L of the test item 1,2,3 -Propanetricarboxylic acid, 2 -Hydroxy-, tris(C12 -C13 -branched-alkyl)ester were determined according to OECD 202 (2004) from 2015-06-01 to 2015-06-05, with the definitive exposure phase from 2015-06-03 to 2015-06-05. The limit test was conducted under static conditions over a period of 48 hours. The test item is a clear and colourless liquid with a negligible solubility in water. Therefore, a saturated solution with a nominal loading of 100 mg/L of the test item was prepared with ISO test water (acc. to OECD 202, Annex 3) by stirring with approximately 1100 rpm for 48 hours at room temperature followed by filtration with a membrane filter (0.2 µm, RC, MACHEREY-NAGEL) to achieve the saturated solution. The saturated solution was visually clear throughout the exposure phase of 48 hours. Twenty daphnids each were exposed to the limit concentration and the control. The concentrations of 1,2,3 -Propanetricarboxylic acid, 2-Hydroxy-, tris(C12-C13-branched-alkyl)ester were analytically verified via LC-MS/MS at the start of the exposure (0 hours) and at the end of the exposure (48 hours) in the saturated solution and the control. With regard to the measured concentrations in samples of the non GLP preliminary range finding test with measured concentrations of 60 µg/L and sensitivity of the analytical method, the limit of quantification (LOQ) was set to 40 µg test item /L. However, the measured concentrations of the test item at the start (0 hours) and at the end of the exposure (48 hours) were below LOQ in the definitive study. This can be attributed to the fact, that the water solubility of the test item is very low (≤ 0.739 ng/L), lower than the LOQ. Since no effects on daphnia magna were observed, the analytical results were acceptable. The effect levels given are based on the nominal loading of the test item 1,2,3 -Propanetricarboxylic acid, 2 -Hydroxy-, tris(C12-C13-branched-alkyl)ester. The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 hours) and at the end of the exposure (48 hours), were within the acceptable limits. The validity criteria of the test guideline were fulfilled. At the saturated solution with a nominal loading of 100 mg/L of the test item 1,2,3-Propanetricarboxylic acid, 2-Hydroxy-, tris(C12-C13-branched-alkyl)ester, no effects were observed on Daphnia magna. Thus, the test item is not toxic in the range of the water solubility.

Description of key information

In acute immobilisation tests with Daphnia magna (STRAUS), the effects of a saturated solution of the similar substance 1,2,3 -Propanetricarboxylic acid, 2 -hydroxy-, tris(C12 -13 -branched-alkyl) ester and the source substance Tri-(hexyl, octyl, decyl) citrate resulted in no toxicity up to the range of water solubility. The same is expected for 1,2,3-Propanetricarboxylic acid, 2-hydroxy-, tri-C14-15-alkyl esters (Please see Analogue Approach).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of a saturated solution with a nominal loading of 100 mg/L of the test item 1,2,3 -Propanetricarboxylic acid, 2 -Hydroxy-, tris(C12 -C13 -branched-alkyl)ester were determined according to OECD 202 (2004) from 2015-06-01 to 2015-06-05, with the definitive exposure phase from 2015-06-03 to 2015-06-05. The limit test was conducted under static conditions over a period of 48 hours. The test item is a clear and colourless liquid with a negligible solubility in water. Therefore, a saturated solution with a nominal loading of 100 mg/L of the test item was prepared with ISO test water (acc. to OECD 202, Annex 3) by stirring with approximately 1100 rpm for 48 hours at room temperature followed by filtration with a membrane filter (0.2 µm, RC, MACHEREY-NAGEL) to achieve the saturated solution. The saturated solution was visually clear throughout the exposure phase of 48 hours. Twenty daphnids each were exposed to the limit concentration and the control. The concentrations of 1,2,3 -Propanetricarboxylic acid, 2-Hydroxy-, tris(C12-C13-branched-alkyl)ester were analytically verified via LC-MS/MS at the start of the exposure (0 hours) and at the end of the exposure (48 hours) in the saturated solution and the control. With regard to the measured concentrations in samples of the non GLP preliminary range finding test with measured concentrations of 60 µg/L and sensitivity of the analytical method, the limit of quantification (LOQ) was set to 40 µg test item /L. However, the measured concentrations of the test item at the start (0 hours) and at the end of the exposure (48 hours) were below LOQ in the definitive study. This can be attributed to the fact, that the water solubility of the test item is very low (≤ 0.739 ng/L), lower than the LOQ. Since no effects on daphnia magna were observed, the analytical results were acceptable. The effect levels given are based on the nominal loading of the test item 1,2,3 -Propanetricarboxylic acid, 2 -Hydroxy-, tris(C12-C13-branched-alkyl)ester. The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 hours) and at the end of the exposure (48 hours), were within the acceptable limits. The validity criteria of the test guideline were fulfilled. At the saturated solution with a nominal loading of 100 mg/L of the test item 1,2,3-Propanetricarboxylic acid, 2-Hydroxy-, tris(C12-C13-branched-alkyl)ester, no effects were observed on Daphnia magna. Thus, the test item is not toxic in the range of the water solubility.