3,7-dimethylocta-2,6-dienyl acetate

Short-term toxicity to fish

Description of key information

For Neryl acetate multi no acute fish toxicity is available. For a close analogue Linalyl acetate such information is present, which can be used for read across to fill this data gap. Below the fish toxicity of Linalyl acetate is summarised. The read across rationale is presented in the Aquatic toxicity Endpoint summary, together with Daphnia and algae. The accompanying files are also attached there.

Linalyl acetate and short-term fish toxicity

The acute toxicity of Linalyl acetate to fish was examined in a study according to OECD TG 203, and in compliance with GLP criteria. In this study, groups of 7 fish (C. carpio) were exposed to nominal tests substance concentrations of 0 (control), 10, 18, 32, 56, and 100 mg/L for 96 hours under flow-through conditions. Samples for analytical confirmation of actual test concentrations were taken at the start and the end of the test period. The actual concentrations at the start were 8.2, 12.4, 20.1, 27.3 and 27.2 mg/L. At the end of the test period, only the nominal test concentrations of 10 and 18 mg/L, were analysed as at higher concentrations, all fish had died before the end of the test. Only for the lowest two nominal concentrations were average concentrations calculated, and these were determined to be 7.9, and 12.3 mg/L, respectively. These concentrations remained stable within ± 20% window during the test. The other three test concentrations were expressed as initial measured concentrations, but based on stability at lower concentrations, treated as average concentration for calculation of effect values. A visible test substance film appeared on the surface of the test solutions at nominal test concentrations of 32 to 100 mg/L, which could not be prevented completely due to the limited solubility of the test substance in the test medium. Fish mortality and other effects were recorded at ca. 2, 20, 44, 72, and 96 hours after the start of the test. The test was valid.

Results: No mortality was noted at a measured test concentration of 7.9 mg/L. At the test concentration of 12.3 mg/L, 5 fish (71%) had died after the 96 hour exposure period. At all higher test concentrations, fish died within 20 hours after the start of exposure. Sub-lethal effects included, hypoactive swimming and loss of equilibrium starting at 46 hours at a concentration of 10 mg/L (nominal) and narcotic effects at concentrations of 12 mg/L and higher. Based on these findings, the 96-h LC50 was determined to be 11 mg/L (95% C.I. 10-14 mg/L), expressed as measured concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

11 mg/L