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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Samples were taken at 0, 96 h and 24 h, 48 h, 72 h (before and after test solution renewal)
Vehicle:
yes
Remarks:
ultrapure water
Test organisms (species):
other: Gobiocypris rarus (rare minnow)
Details on test organisms:
TEST ORGANISM
- Common name: rare minnow
- Source: bred and cultured in the laboratory
- Length at study initiation (length definition, mean, range and SD): 2.0-4.0 cm

ACCLIMATION
- Acclimation period: 7 day
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: feeding until 24 h before test
- Health during acclimation (any mortality observed): no abnormalities noted

FEEDING DURING TEST : no
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
10-250 mg CaCO3/L
Test temperature:
21-25 °C
pH:
6.0 - 8.5
Dissolved oxygen:
>80% of air saturation value
Nominal and measured concentrations:
Nominal: 10 mg/L
Measured: 92.81 - 129.44 µg/L
Reference substance (positive control):
not required
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 43.6 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Details on results:
- Behavioural abnormalities:
- Observations on body length and weight: no effects
- Other biological observations: none
- Mortality of control: no effects
- Other adverse effects control: no effects
- Abnormal responses:no effects
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no effects
- Effect concentrations exceeding solubility of substance in test medium: no
Validity criteria fulfilled:
yes
Remarks:
mortality of controls < 10% (0%), dissolved oxygen >60% (66.9-88-2%)
Conclusions:
The 96-h LC50 of Di-PETMP is greater than 10 mg/L (nominal), the highest concentration tested.
Executive summary:

The acute toxicity of the test item Di-PETMP to rare minnow (Gobiocypris rarus) was determined by a semi-static test according to OECD TG 203 . Based on the result of the preliminary test, a
limit test was performed with a nominal concentration of 10mg/L of the test item (Solubility of Di-PETMP in water: </= 3.7 mg/L). A blank control was carried out at the same time. The test duration was 96h and the frequency of renewal in the limit test was every 24h.
Seven test organisms were used for test concentration and control, respectively. No replicate was used. During the test, the mortality and abnormal behavior of the test
organism were recorded at 3h, 24h, 48h, 72h, 96h and the 96h-LCs, was determined.
Water quality parameters of test solution including temperature, dissolved oxygen and pH value were measured during the test duration. The test concentrations of test solutions were analyzed by LC-MS/MS.


There were no mortalities or abnormalities observed in fish of the control group and the treatment group during the test.
Concentration analysis result of samples showed that, for newly prepared saturated test solution with nominal concentration of 10mg/L, the averaged analyzed concentration were 92.81 µg/L, 94.85 µg/L, 104.36 mg/L, 129.44 µg/L at time point of 0h, 24h, 48h and 72h (new solution ), respectively; After 24 hour of exposure, the analyzed concentrations at time point of 24h, 48h, 72h and 96h (old solution ) were all less than the limit of quantification (LOQ,50m g/L). During the test, the analyzed concentrations of Di-PETMP were not maintained in the initial concentration of 80%-120% in the exposure system because of the inevitable oxidation effect.


However, effect levels based on mean measured concentrations don't represent the real environmental conditions because the test substance is rapidly oxidized by the oxygen content in the aqueous phase. Therefore, nominal values are used for hazard assessment.

Description of key information

The 96-h LC50 of Di-PETMP is greater than 10 mg/L(nominal), the highest concentration tested.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
> 10 mg/L

Additional information

The acute toxicity of the test item Di-PETMP to rare minnow (Gobiocypris rarus) was determined by a semi-static test according to OECD TG 203 . Based on the result of the preliminary test, a
limit test was performed with a nominal concentration of 10mg/L of the test item (Solubility of Di-PETMP in water: </= 3.7 mg/L). A blank control was carried out at the same time. The test duration was 96h and the frequency of renewal in the limit test was every 24h.
Seven test organisms were used for test concentration and control, respectively. No replicate was used. During the test, the mortality and abnormal behavior of the test
organism were recorded at 3h, 24h, 48h, 72h, 96h and the 96h-LCs, was determined.
Water quality parameters of test solution including temperature, dissolved oxygen and pH value were measured during the test duration. The test concentrations of test solutions were analyzed by LC-MS/MS.


There were no mortalities or abnormalities observed in fish of the control group and the treatment group during the test.
Concentration analysis result of samples showed that, for newly prepared saturated test solution with nominal concentration of 10mg/L, the averaged analyzed concentration were 92.81 µg/L, 94.85 µg/L, 104.36 mg/L, 129.44 µg/L at time point of 0h, 24h, 48h and 72h (new solution ), respectively; After 24 hour of exposure, the analyzed concentrations at time point of 24h, 48h, 72h and 96h (old solution ) were all less than the limit of quantification (LOQ,50m g/L). During the test, the analyzed concentrations of Di-PETMP were not maintained in the initial concentration of 80%-120% in the exposure system because of the inevitable oxidation effect.


However, effect levels based on mean measured concentrations don't represent the real environmental conditions because the test substance is rapidly oxidized by the oxygen content in the aqueous phase. Therefore, nominal values are used for hazard assessment.