sucrose laurate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 10-13, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Test material form:

solid: granular

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No. of test material: Mitsubishi-Chemical Foods Corporation; 6912911B
- Expiration date of the lot/batch: September 11, 2018
- Purity test date: June 27, 2017

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: below 15 degrees C in a cool, dark, well-ventilated space with the container tightly closed
- Solubility and stability of the test substance in the solvent/vehicle: forms highly viscous solution or gel when mixed with water

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution was prepared by adding 315.999 mg of test item to a 1 L bottle. 1 L of growth medium was added, and the solution stirred for 2 hrs at room temperature.

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM

- Source (laboratory, culture collection): SAG, Culture Collection of Algae at Pflanzenphysiologishces Institut of the University of Gottingen, Albrecht von Haller Institut, Untere Klarspule 2, D-37073 Gottingen, Catalog No 61.81 SAG

ACCLIMATION
- Acclimation period: 4 days
- Culturing media and conditions (same as test or not): same

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22 degrees C

pH:

7.73-8.06

Nominal and measured concentrations:

Nominal: 0, 3.16, 10.0, 31.6, 100, 316 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass flasks
- Type (delete if not applicable): open (air-permeable silicone-sponge caps)
- Material, size, headspace, fill volume: glass, 250 mL fill volume
- Aeration: no
- Initial cells density: 10,000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 8


GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified water

Reference substance (positive control):

yes

Remarks:

The sensitivity of the test organism is checked twice a year using 3,5-dichlorophenol.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

133 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): Yes, at 72 hrs first cell debris was visible at all test concentrations.

Reported statistics and error estimates:

ErC50 95%-cl 1243-144 mg/L
EyC50 95%-cl 50.7-61.3 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 72-hr EC50 based on growth rate was 133 mg/L.

Executive summary:

The toxicity to alga of the test substance was determined in an OECD Guideline 201 test. Algae were exposed to 0, 3.16, 10.0, 31.6, 100, 316 mg/L of test substance. Due to difficulties analyzing the test substance, nominal concentrations were reported. The 72 hr EC50 based on growth rate was 133 mg/L, with an NOEC of 10 mg/L.