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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-pyridylamine
EC Number:
207-987-9
EC Name:
4-pyridylamine
Cas Number:
504-24-5
Molecular formula:
C5H6N2
IUPAC Name:
nickel
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test media.

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
Test system: rotary shaking culture (ca. 100 times/min)

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
not specified
Test temperature:
21 - 24 degreeC (variation range : +/- 2 degreeC)
pH:
not specified
Dissolved oxygen:
not specified
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
60.0, 27.3, 12.4, 5.63, 2.56, 1.16 mg/L (geometric ratio 2.2) and control
Details on test conditions:
TEST SYSTEM: - Test system: rotary shaking culture (ca. 100 times/min), - Material, size, headspace, fill volume: test solution volume: 600 mL/control (100 mL/vessel), 300 mL/concentration (100 mL/vessel), - No. of vessels per concentration (replicates): 3, - No. of vessels per control (replicates): 6, OTHER TEST CONDITIONS: - Light intensity and quality: lighting by fluorescent lamp [continuous lighting, light intensity near the liquid surface was 60 - 120 microE/m2/s (variation range; +/-20 %)], EFFECT PARAMETERS MEASURED: - Measurement of growth: cell concentration
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
12.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
5.63 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass

Any other information on results incl. tables

Rate of concentrations in the test solution for nominal values:

at the start of exposure: 98.9 - 100 %,

at the end of exposure: 98.4 - 101 %.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
EC50(72h) 30 mg/l
Executive summary:

EC50(72h) 30 mg/l