1-Ethoxy-2,3-difluoro-4-[4-(trans-4-pentylcyclohexyl)-1-cyclohexene-1-yl]-benzene

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 16, 2013 - December 31, 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

2008

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3100 (Aerobic Aquatic Biodegradation)

Version / remarks:

1998

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,3-difluoro-1-propoxy-4-[4-(trans-4-propylcyclohexyl)-1-cyclohexen-1-yl]-benzene

EC Number:

600-065-0

Cas Number:

1003218-40-3

Molecular formula:

C24H34F2O

IUPAC Name:

2,3-difluoro-1-propoxy-4-[4-(trans-4-propylcyclohexyl)-1-cyclohexen-1-yl]-benzene

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Nanjing Chengdong Sewage Treatment Plant, treating predominantly domestic sewage
- Preparation of inoculum for exposure: Sludge was washed and centrifuged at 4°C. Washing procedure repeated 3 times.
- Concentration of sludge: 4 g/L dw
- Initial cell/biomass concentration: 30 mg(L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

9 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: based salt medium
- Test temperature: 22 +- 1°C
- pH: 7.96 - 8.21

TEST SYSTEM
- Culturing apparatus: glass bottles 500 mL
- Number of culture flasks/concentration: 2

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- Toxicity control: 1 flask

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

< 1

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

76.0% in 14 days

Any other information on results incl. tables

Inoculum blank: O2 uptake 19.9 mg/L in 28 d

Toxicity control: 37.3% in 14 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The ready biodegradabilityh of the test item was determined in a study according to OECD 301F (Manometric Respirometry Test). The biodegradation was determined to be <1% after 28 d. Biodegradation of the reference substance and the results of the controls were within the validity criteria. Therefore, the test item has to be considered as not readily biodegradable under the conditions of this test.