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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1977
Report date:
1977

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: FDA-Guideline; Federal Register Vol. 38, Nr. 187, page 27019, year 1973
GLP compliance:
no

Test material

Specific details on test material used for the study:
Colourless crystalline material.

Test animals / tissue source

Species:
rabbit
Strain:
Himalayan
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: HOECHST AG
- Weight at study initiation: 1.5 - 2.0 kg
- Housing: individually
- Diet: ERKA 8300 (Futtermittelwerke Robert Koch oHG/Hamm), ad libitum
- Water: ad libitum

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes
Amount / concentration applied:
100 mg undiluted
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
72 hours
Number of animals or in vitro replicates:
6
Details on study design:
Eyes were rinsed with physiological saline 24 hours after instillation.
Eyes were inspected for integrity before the testing. A solution of fluorescein, sodium salt (0.01%)
was dropped into the eye. Then the cornea was investigated using UV radiation 24 hours before app
lication. Afterwards eyes were rinsed.
24 and 72 hours after application eyes were examined for integrity in the same manner.

Results and discussion

In vivo

Irritant / corrosive response data:
After application of the undiluted test material, a severe swelling of the conjunctiva (eyelids completely closed) was noted in all rabbits.
An assessment of the cornea, iris and redness of the conjunctiva was therefore not possible.

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
Based on the results of this test, 1,2-Bis(chloromethyl)benzene has to be regarded as a strong irritant to the eye.