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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
A comparison of the acute toxicity of two forms of thiamine
Author:
Haley, T.J.
Year:
1948
Bibliographic source:
Proc Soc Exp Biol Med. 68:153-157

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 22 - 42 g

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
not specified
Doses:
380, 385, 390, 395 and 400 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no

Results and discussion

Effect levels
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
387.3 mg/kg bw
Based on:
test mat.
Remarks on result:
other: LD50 derivation according to the method of Miller and Tainter; range > 385 < 390 mg/kg bw
Mortality:
390 mg/kg bw: 1/5 animals died
385 mg/kg bw: 3/5 animals died
390 mg/kg bw: 2/5 animals died
395 mg/kg bw: 4/5 animals died
400 mg/kg bw: 3/5 animals died
Clinical signs:
Symptoms of toxicity, such as restlessness, labored respiration, vasodilatation, cynosis, muscular twitching, clonic convulsions and death by respiratory paralysis occurred. In addition, visual signs of anoxia, gradually deepening bluish coloration of the ears and all other body areas where the fur was thin enough to permit direct observation, was observed.
Gross pathology:
In all animals cardiac arrhythmias were seen after opening the thoracic cage.

Applicant's summary and conclusion