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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999-11-15 to 1999-12-07
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
Part I; 1984
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
1992
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Version / remarks:
1996
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
The test medium of the highest test concentration of nominal 100 mg/L was prepared by dissolving 50 mg test substance into 500 mL test water by intense stirring for 10 minutes. Adequate volumes of this test medium were diluted with test water to prepare the desired test media. The test media were prepared just before introduction of Daphnia (= start of the test).

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Straus), clone 5
- Sex: female
- Source: supplied 1997 by the Umweltbundesamt, Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age at study initiation (mean and range, SD): 6.5 - 23.5 hours old
- Method of breeding: The Daphnia were bred in laboratories under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test. The test organisms were not first brood progeny.

ACCLIMATION
- Acclimation period: for 6.5 hours under test conditions
- Acclimation conditions (same as test or not): yes, same

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
Water hardness of reconstituted water: 250 mg/L CaCO3
Test temperature:
21°C
pH:
7.4 to 8.0
Dissolved oxygen:
8.1 - 8.6 mg/L
Salinity:
not applicable
Nominal and measured concentrations:
Nominal concentrations: 4.6, 10, 21, 46 and 100 mg/L
All biological results are related to nominal concentrations of the test substance.
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL glass beakers with 50 ml test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16h light : 8h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilty, after 24 and 48h
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Any other information on results incl. tables

Dissolved oxygen concentrations and pH-values in the test media at test start and end of the test

concentration
[mg/L]
start (0h) end (48h)
pH oxygen
[mg/L]
pH oxygen
[mg/L]
control 7.9 8.3 7.8 8.3
4.6 8.0 8.3 7.8 8.4
10 8.0 8.3 7.8 8.2
21 7.8 8.2 7.8 8.1
46 7.6 8.1 7.8 8.2
100 7.4 8.1 7.8 8.6

Influence of the test item on the mobility of Daphnia magna

concentration
[mg/L]
No. of Daphnia tested No. Of immobilized Daphnia after % of immobilized Daphnia after
24h 48h 24h 48h
control 20 0 0 0 0
4.6 20 0 0 0 0
10 20 0 0 0 0
21 20 0 0 0 0
46 20 0 0 0 0
100 20 0 0 0 0

No immobilization of the test animals was observed in the control and all test concentrations up to 100mg/L.

The 48h LOEC and EC50 are therefore > 100mg/L.

The 48 h NOEC and EC0 of teh test item to Daphnia magna were determined to be at least 100 mg test substance/L. The NOEC and EC0 might even be higher than this concentration, but concentrations in excess of 100 mg test substance/L have not been tested.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A 48-hour EC50 value of > 100 mg/L has been determined for the effects of the test substance on mobility of Daphnia magna.