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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
21 Jul - 18 Aug 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1-ethyl-2-[1,2,3,4-tetrahydro-1-(2-hydroxyethyl)-2,2,4-trimethyl-6-quinolyl]benz[cd]indolium chloride
EC Number:
264-497-8
EC Name:
1-ethyl-2-[1,2,3,4-tetrahydro-1-(2-hydroxyethyl)-2,2,4-trimethyl-6-quinolyl]benz[cd]indolium chloride
Cas Number:
63817-45-8
Molecular formula:
C27H31N2O.Cl
IUPAC Name:
2-ethyl-3-[1-(2-hydroxyethyl)-2,2,4-trimethyl-1,2,3,4-tetrahydroquinolin-6-yl]-2-azatricyclo[6.3.1.0^{4,12}]dodeca-1(11),2,4(12),5,7,9-hexaen-2-ium chloride
Test material form:
liquid
Details on test material:
Basic Blue 147 Cl

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Wupperverband (Germany), sampling date 15 Jul 1994
- Pretreatment: none
- Concentration of sludge: 5 mL/L
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
9.6 mg/L
Based on:
test mat.
Initial conc.:
847 other: mg O2/g
Based on:
COD
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Blank control performed in parallel

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
8
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The substance showed a maximum biodegradation rate of 8% (based on O2 consumption). The substance is not considered to be readily biodegradable.