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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
03 Jan 1991 - 31 Jan 1991 (experimental)
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
only draft report available
Reference:
Composition 0
Composition 0
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes
Test material information:
Composition 1
Oxygen conditions:
aerobic
Inoculum or test system:
other: supernatant filtered from activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sewage sludge bacteria from the aeration stage of a sewage plant treating predominantly domestic sewage
- Preparation of inoculum for exposure: the sample was allowed to settle and the supernatant filtered through a Whatman GFA filter paper (first 250 mL discarded)
Duration of test (contact time):
28 d
Initial conc.:
3 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 20 +/- 1 °C


TEST SYSTEM
- Number of culture flasks/concentration: 2


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (O2 consumption)
Value:
> 80 - < 90
Sampling time:
28 d
Details on results:
Kinetic of test substance (in %):
= 56 after 5 days
= 64 after 15 days
= 81 after 28 days
Results with reference substance:
88% degradation after 28 days

Under the conditions, of the test, acrylic acid was readily biodegradable according to OECD criteria (14 -day window criterion as given in OECD test guideline fulfilled).

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Under the conditions, of the test, acrylic acid was readily biodegradable according to OECD criteria (14 d window criterion as given in OECD test guideline fulfilled). The degree of biodegradation reached 80-100% within 14 d.
Executive summary:

A study was conducted to determine readily biodegradability of the test substance according to OECD Guideline 301D. Under the conditions, of the test, acrylic acid was readily biodegradable according to OECD criteria (14 d window criterion as given in OECD test guideline fulfilled). The degree of biodegradation reached 80-100% within 14 d (BAMM, 1991).

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
31 May 1995 - 09 June 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 0
Composition 0
Qualifier:
according to
Guideline:
EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
Version / remarks:
92/69/EEC, part II C 4-A
GLP compliance:
yes
Test material information:
Composition 1
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge taken from a municipal waste water treatment plant (Marl-Ost, Germany).
- Concentration of sludge: 3.8 g/l
Duration of test (contact time):
9 d
Initial conc.:
15.08 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 21.8 - 22 °C

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Measuring equipment: Shimadzu

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Reference control: yes
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (DOC removal)
Value:
> 90 - < 100
Sampling time:
9 d
Details on results:
Kinetic of test substance (in %):
= 2 after 2 days
= 92 after 6 days
= 94 after 7 days
= 95 after 9 days
Results with reference substance:
Degradation >70 % within 14 days

Under the conditions of the test, acrylic acid was readily biodegradable according to OECD criteria (10 -day window fulfilled).

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Under the conditions of the test, acrylic acid was readily biodegradable according to OECD criteria (10 d window fulfilled). The degree of biodegradation reached 90-100% within 9 d.
Executive summary:

A study was conducted to determine readily biodegradability of the test substance according to EU Method C.4-A. Under the conditions of the test, acrylic acid was readily biodegradable according to OECD criteria (10 d window fulfilled). The degree of biodegradation reached 90-100% within 9 d (Huels, 1995).

Endpoint:
biodegradation in water: ready biodegradability
Data waiving:
study technically not feasible
Justification for data waiving:
the study does not need to be conducted because the substance is inorganic

Description of key information

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

No biodegradation testing with zinc is required as the substance is inorganic.

Acrylic acid studies

Study 1:

A study was conducted to determine readily biodegradability of the test substance according to EU Method C.4-A. Under the conditions of the test, acrylic acid was readily biodegradable according to OECD criteria (10 d window fulfilled). The degree of biodegradation reached 90-100% within 9 d (Huels, 1995).

Study 2:

A study was conducted to determine readily biodegradability of the test substance according to OECD Guideline 301D. Under the conditions, of the test, acrylic acid was readily biodegradable according to OECD criteria (14 d window criterion as given in OECD test guideline fulfilled). The degree of biodegradation reached 80-100% within 14 d (BAMM, 1991).

[Type of water: freshwater]