1-cyclohexyl-1H-pyrrole-2,5-dione

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 April to 09 May 1985

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Deviations:

not specified

GLP compliance:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Nippon Shokubai Kagaku Kogyo Co., Ltd./ No. C-2050
- Expiration date of the lot/batch: no data
- Purity test date: no data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

TEST CONDITIONS
- Composition of medium: conventional activated sludge
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature: 25 ± 1°C
- pH: 6.2
- pH adjusted: no
- CEC (meq/100 g):
- Aeration of dilution water: no
- Suspended solids concentration: no data
- Continuous darkness: yes/no
- Other:

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:

STATISTICAL METHODS:

Reference substance:

not required

Key result

Parameter:

% degradation (test mat. analysis)

Value:

82

Sampling time:

28 d

HPLC revealed the test substance was eliminated in the (sludge + test substance) systems and dramatically decreased in the (water + test substance) system, indicating the test substance undergoes degradation and remains as degradation product in water.

Comparison of HPLC retention time and mass spectrum using N-cyclohexylmaleamidic acid (predicted degradation product) reference standard confirmed the formation of N-cyclohexylmaleamidic acid.

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

Under the conditions of this study N-Cyclohexylmaleimide was found to be readily biodegradable.

Description of key information

Under the conditions of this study N-Cyclohexylmaleimide was found to be readily biodegradable.  

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater