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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP study with limited information on test material, test animals, study design and clinical examination

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
yes
Remarks:
limited test material, testing animal and study design information, only 50 mg was applied to the animals which is not guideline-compliant
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Naphthalene-1,5-disulphonic acid
EC Number:
201-317-9
EC Name:
Naphthalene-1,5-disulphonic acid
Cas Number:
81-04-9
Molecular formula:
C10H8O6S2
IUPAC Name:
naphthalene-1,5-disulfonic acid
Test material form:
solid: crystalline
Details on test material:
Unspecified test material

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: the untreated eye served as control
Duration of treatment / exposure:
single application of 0.05 g without washing
Observation period (in vivo):
7 days
Reading time points: 24, 48 and 72 h and 7 days
Number of animals or in vitro replicates:
2 (1 male, 1 female)

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
iris score
Basis:
animal #1
Time point:
other: mean of 24, 48 and 72 h
Score:
1.33
Max. score:
2
Reversibility:
other: not reversible within 7 days
Irritation parameter:
iris score
Basis:
animal #2
Time point:
other: mean of 24, 48 and 72 h
Score:
2
Max. score:
2
Reversibility:
other: not reversible within 7 days
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
other: mean of 24, 48 and 72 h
Score:
2
Max. score:
3
Reversibility:
other: not reversible within 7 days
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
other: mean of 24, 48 and 72 h
Score:
3
Max. score:
3
Reversibility:
other: not reversible within 7 days
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
other: mean of 24, 48 and 72 h
Score:
2
Max. score:
4
Reversibility:
other: not reversible within 7 days
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
other: mean of 24, 48 and 72 h
Score:
3
Max. score:
4
Reversibility:
other: not reversible within 7 days
Other effects:
The test material was highly irritating and caused corrosion on the cornea of the rabbit eyes when applied at a dose level of 50 mg.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Executive summary:

The test material was highly irritating in an in viov study and caused corrosion on the cornea of the rabbit eyes when applied at a dose level of 50 mg.