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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental result performed using standard methods of analysis
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound on the Zebra fish(Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The experimental phase of the main study started at 23-01-2018 and lasted for a period of 96 hrs.
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material (IUPAC name): (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one- Common name: d-Carvone- Molecular formula: C10H14O- Molecular weight: 150.22 g/mol- Smiles notation: C1[C@H](CC=C(C1=O)C)C(C)=C- InChl: 1S/C10H14O/c1-7(2)9-5-4-8(3)10(11)6-9/h4,9H,1,5-6H2,2-3H3/t9-/m0/s1- Substance type: Organic- Physical state: colorless liquid-Purity:99.52%-Solubility in Water:1879.65 mg/L
Analytical monitoring:
yes
Details on sampling:
-Storage Conditions:Ambient Temp (23 to 27 °C)
Vehicle:
yes
Remarks:
potable water
Details on test solutions:
No data available
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name: Zebra fish- Source:Anytime Pet,Jaripatka, Nagpur- Length at study initiation :average 1.78 cm- Weight at study initiation :0.080g (average)ACCLIMATION- Acclimation period: 7 days- Type and amount of food during acclimation:Standard brand feed- Feeding frequency during acclimation:Daily once
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
No mortailty was observed till 48 hrs
Hardness:
150.5
Test temperature:
23 °C
pH:
7.2
Dissolved oxygen:
7.0 mg/l
Nominal and measured concentrations:
6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
Details on test conditions:
TEST SYSTEM - Test vessel: plastic aquaria - fill volume: 5 liter - No. of organisms per vessel:8-Areation:Aeration in test vessels was provided 1 day before the start of experiment
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

8.1. Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

6.25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

12.5

4

4

-

-

-

-

-

-

-

-

-

-

-

-

25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

2

2

2

2

2

2

2

2

100

4

4

2

2

4

4

4

4

4

4

4

4

4

4

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

6.25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

12.5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

50

4

4

NS4

NS4

NS4

NS4

M2

M2

M2

M2

M2

M2

M2

M2

100

4

4

M2

M2

-

-

-

-

-

-

-

-

-

-

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.5

7.5

7.7

7.7

7.9

7.9

8.0

8.0

6.25

4

4

7.7

7.7

7.8

7.8

8.0

8.0

8.1

8.1

12.5

4

4

7.7

7.7

7.8

7.8

8.0

8.0

8.3

8.3

25

4

4

7.7

7.7

7.9

7.9

8.0

8.0

8.3

8.3

50

4

4

7.8

7.8

7.9

7.9

8.1

8.1

8.3

8.3

100

4

4

-

-

-

-

-

-

-

-

Nominal

Concentration

(mg/l)

Number of

fish

Oxygen content (mg/L) after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.9

6.9

6.8

6.8

6.7

6.7

6.6

6.6

6.25

4

4

6.8

6.8

6.7

6.7

6.6

6.6

6.5

6.5

12.5

4

4

6.8

6.8

6.7

6.7

6.6

6.6

6.5

6.5

25

4

4

6.8

6.8

6.7

6.7

6.6

6.6

6.5

6.5

50

4

4

6.8

6.8

6.7

6.7

6.6

6.6

6.5

6.5

100

4

4

-

-

-

-

-

-

-

-

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.2244-16-8 on Zebra fish(Danio rerio)was found to be 50 mg/L.
Executive summary:

A 96 h study was conducted for CAS No. 2244-16-8 on Zebra fish(Danio rerio), at a test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 50 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 25 mg/L

LC50 (96 hours) Experimental = 50 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100 mg/L.

Based on the above effect concentration (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one can be classified as aquatic chronic 3 .

Eventhough the experimental result is considered (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one is toxic for fish but

(5S)-2-methyl-5- (prop-1-en-2-yl)cyclohex-2-en-1-one is readily biodegradable . Hence it can be considered Not classified as per CLP classification.

Description of key information

A 96 h study was conducted for CAS No. 2244-16-8 on Zebra fish(Danio rerio), at a test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 50 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 25 mg/L

LC50 (96 hours) Experimental = 50 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100 mg/L.

Based on the above effect concentration (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one can be classified as aquatic chronic 3 .

Eventhough the experimental result is considered (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one is toxic for fish but

(5S)-2-methyl-5- (prop-1-en-2-yl)cyclohex-2-en-1-one is readily biodegradable . Hence it can be considered Not classified as per CLP classification.

Key value for chemical safety assessment

LC50 for freshwater fish:
50 mg/L

Additional information

The experimental study was conducted by NS Support Services to evaluate short teerm toxicity to fish of test chemical (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one

A 96 h study was conducted for CAS No. 2244-16-8 on Zebra fish(Danio rerio), at a test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 50 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 25 mg/L

LC50 (96 hours) Experimental = 50 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100 mg/L.

Based on the above effect concentration (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one can be classified as aquatic chronic 3 as per CLP classification.

Eventhough the experimental result is considered (5S)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one is toxic for fish but

(5S)-2-methyl-5- (prop-1-en-2-yl)cyclohex-2-en-1-one is readily biodegradable . Hence it can be considered Not classified as per CLP classification.