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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Related information:
Composition 1
Reason / purpose:
read-across source
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on toxicity study of microorganism for the test chemical :
1.The Microtox acute toxicity assay was performed by using a modified strain of Vibrio fischeri
2.To evaluate toxicity of the test material on the basis of inhibition of the light output of Vibrio fisheri
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material (IUPAC name): 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid
- Molecular formula: C17H14N2O8S2
- Molecular weight: 438.4356 g/mol
- Smiles notation: c1cc(cc(c1)N)C(=O)Nc2ccc(c3c2c(cc(c3)S(=O)(=O)O)O)S(=O)(=O)O
-InChl:1S/C17H14N2O8S2/c18-10-3-1-2-9(6-10)17(21)19-13-4-5-15(29(25,26)27)12-7-11(28(22,23)24)8-14(20)16(12)13/h1-8,20H,18H2,(H,19,21)(H,22,23,24)(H,25,26,27)
- Substance type: Organic
- physical state: solid
Details on sampling:
1. Sample storage conditions before analysis: Frozen samples were brought to room temperature, and centrifuged.
Vehicle:
not specified
Test organisms (species):
Vibrio fisheri
Details on inoculum:
Details on test organisms
Laboratory culture: From eight serial dilutions, the percent concentration to decrease 20% of the luminescence of amodified strain of Vibrio fischeri
(EC20) after 5 min incubation was calculated with the Microtox data analysis program
Method of cultivation: No data
Preparation of inoculum for exposure: Frozen samples were brought to room temperature, and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH. Colour correction was done at 490 nm.
Pretreatment: No data
Initial biomass concentration: No data
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
5 min
pH:
The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH
Details on test conditions:
1.TEST SYSTEM
Test vessel: Microtox 500 Analyzer
No. of vessels per concentration (replicates): triplicate analysis.

OTHER TEST CONDITIONS
Adjustment of pH: The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH
Photoperiod:
Light intensity: Colour correction was done at 490 nm.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The percent concentration to decrease 20% of the luminescence of amodified strain of Vibrio fischeri (EC20) after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software

Reference substance (positive control):
yes
Remarks:
1. A solution of 1 g/l ZnSO4.7H2O was used as the positive control
Duration:
5 min
Dose descriptor:
other: EC20
Effect conc.:
44.6 other: dilution
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: Read across 1
Duration:
5 min
Dose descriptor:
EC50
Effect conc.:
22.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: inhibition of light output
Remarks on result:
other: Read across 2
Results with reference substance (positive control):
2) EC50 refers to the concentration of compound (mg/L) required to inhibit the light output of V. fischeri by 50% after a 5 min exposure period.
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic to microorganism .
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.The Microtox acute toxicity assay was performed by using a modified strain ofVibrio fischeri. Frozen samples were brought to room temperature, and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples before and after decoloration according to the test protocols defined by the manufacturer From eight serial dilutions, the percent concentration to decrease 20% of the luminescence of amodified strain ofVibrio fischeri(EC20)after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software(1999) Azur Environmental, Newark, Del.]. A solution of 1 g/l ZnSO4·7H2O was used as the positive control and 1 g/l glucose as the negative control. Each EC20 reported is the average of triplicate analysis.

The concentration to decrease 50% of the bacterial luminescence in the Microtox acute assay (EC50) is normally reported. However, in most of these studies, the EC50 before or after decoloration was greater than 100% indicating that there was no toxicity or toxicity change. To better evaluate whether the decoloration process affected toxicity, the dilution required to decrease 20% of the bacterial luminescence relative to the control (EC20)was reported instead. The following rating was adapted from Coleman & Qureshi (1985) –

EC20:>100%=nontoxic;

>75–100%=slightly non-toxic;

 >50–75%=toxic;

>25–50%=moderately toxic;

<25% very toxic.

The toxicity of 100mg/l of test material determined in terms of EC20 (% dilution) was 44.6 ± 11.6. According to the ranking scheme for Microtox assay using EC20 values, test material can be categorized under moderately toxic category.

2.Toxicity value of test materila to Vibrio fisheri was found to be EC50: 22.19+/-2.47 on the basis of inhibition of the light output after a 5 min exposure period.

Thus, based on the above summarised studies, Ammonium iodide and it’s structurally and functionally similar read across substance, it can be concluded that

4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is moderately toxic to microorganism.

Description of key information

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.The Microtox acute toxicity assay was performed by using a modified strain ofVibrio fischeri. Frozen samples were brought to room temperature, and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples before and after decoloration according to the test protocols defined by the manufacturer From eight serial dilutions, the percent concentration to decrease 20% of the luminescence of amodified strain ofVibrio fischeri(EC20)after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software(1999) Azur Environmental, Newark, Del.]. A solution of 1 g/l ZnSO4·7H2O was used as the positive control and 1 g/l glucose as the negative control. Each EC20 reported is the average of triplicate analysis.

The concentration to decrease 50% of the bacterial luminescence in the Microtox acute assay (EC50) is normally reported. However, in most of these studies, the EC50 before or after decoloration was greater than 100% indicating that there was no toxicity or toxicity change. To better evaluate whether the decoloration process affected toxicity, the dilution required to decrease 20% of the bacterial luminescence relative to the control (EC20)was reported instead. The following rating was adapted from Coleman & Qureshi (1985) –

EC20:>100%=nontoxic;

>75–100%=slightly non-toxic;

 >50–75%=toxic;

>25–50%=moderately toxic;

<25% very toxic.

The toxicity of 100mg/l of test material determined in terms of EC20 (% dilution) was 44.6 ± 11.6. According to the ranking scheme for Microtox assay using EC20 values, test material can be categorized under moderately toxic category.

2.Toxicity value of test materila to Vibrio fisheri was found to be EC50: 22.19+/-2.47 on the basis of inhibition of the light output after a 5 min exposure period.

Thus, based on the above summarised studies, Ammonium iodide and it’s structurally and functionally similar read across substance, it can be concluded that

4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is moderately toxic to microorganism.

Key value for chemical safety assessment

EC50 or LC50 for microorganisms:
22 mg/L

Additional information

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.The Microtox acute toxicity assay was performed by using a modified strain ofVibrio fischeri. Frozen samples were brought to room temperature, and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples before and after decoloration according to the test protocols defined by the manufacturer From eight serial dilutions, the percent concentration to decrease 20% of the luminescence of amodified strain ofVibrio fischeri(EC20)after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software(1999) Azur Environmental, Newark, Del.]. A solution of 1 g/l ZnSO4·7H2O was used as the positive control and 1 g/l glucose as the negative control. Each EC20 reported is the average of triplicate analysis.

The concentration to decrease 50% of the bacterial luminescence in the Microtox acute assay (EC50) is normally reported. However, in most of these studies, the EC50 before or after decoloration was greater than 100% indicating that there was no toxicity or toxicity change. To better evaluate whether the decoloration process affected toxicity, the dilution required to decrease 20% of the bacterial luminescence relative to the control (EC20)was reported instead. The following rating was adapted from Coleman & Qureshi (1985) –

EC20:>100%=nontoxic;

>75–100%=slightly non-toxic;

 >50–75%=toxic;

>25–50%=moderately toxic;

<25% very toxic.

The toxicity of 100mg/l of test material determined in terms of EC20 (% dilution) was 44.6 ± 11.6. According to the ranking scheme for Microtox assay using EC20 values, test material can be categorized under moderately toxic category.

2.Toxicity value of test materila to Vibrio fisheri was found to be EC50: 22.19+/-2.47 on the basis of inhibition of the light output after a 5 min exposure period.

Thus, based on the above summarised studies, Ammonium iodide and it’s structurally and functionally similar read across substance, it can be concluded that

4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is moderately toxic to microorganism.