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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Related information:
Composition 1
Reason / purpose:
read-across source
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two short term toxicity study of fish for the test chemical :
1.To evaluate short term toxicity of test material on fish
2.To evaluate the acute toxicity of test material on Fathead Minnow fish.
GLP compliance:
not specified
Test material information:
Composition 1
Details on sampling:
2. composite stored a t 20 C in dark.Stock solution refrigerated
Vehicle:
not specified
Test organisms (species):
other: 1.Bruchydunio rerio , 2.Pimephales promelas
Details on test organisms:
2.TEST ORGANISM
- Common name: Fathead Minnow
- Source: Berry water gardens , windmill fish hatcheries , North Carolina
- Length at study initiation : 5.2 cm
- Weight at study initiation: 1.3 g
- Feeding during test: starved 48 hrs before test

ACCLIMATION
- Acclimation period: 10 days
- Acclimation conditions : maintained in plastic stock tank in continuously running charcoal –sand filtered Chapel Hill tap water
- Type and amount of food: protein enriched fish food
- Feeding frequency: 5-7 times per week

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
2) 32mg/l
Test temperature:
2)15°C
pH:
2)6.8-8.5
Nominal and measured concentrations:
2)0, 0.1, 1.8, 3.2, and 5.6, 10.0 mg/l
Details on test conditions:
2. Test vessel: 5-gallon wide mouth glass jar
- No. of organisms per vessel: 10
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
100 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Read across 1
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Read across 2
Details on results:
2. Before death, fish tended to become very slow moving at higher concentration. Dead fish were dyed on heads and fins . Surviving fish were dyed dark green in concentration above 1mg/l
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic to fish atleast in the concentration range 12-100 mg/l
Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.Test material was used to evaluate the short term toxicity onBruchydunio rerio. The effect concentration (EC50 was considered to be less the 100 mg/l.

2.Test material was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

 

10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 12 mg/l . Toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >12 mg/l

Before death, fish tended to become very slow moving at higher concentration. Dead fish were dyed on heads and fins . Surviving fish were dyed dark green in concentration above 1mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 12 -100 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for short term toxicity of fish as " acute Aquatic 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the concentration range 12 -100mg/L .

Description of key information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.Test material was used to evaluate the short term toxicity onBruchydunio rerio. The effect concentration (EC50 was considered to be less the 100 mg/l.

2.Test material was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

 

10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 12 mg/l . Toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >12 mg/l

Before death, fish tended to become very slow moving at higher concentration. Dead fish were dyed on heads and fins . Surviving fish were dyed dark green in concentration above 1mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 12 -100 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for short term toxicity of fish as " acute Aquatic 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the concentration range 12 -100mg/L .

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.Test material was used to evaluate the short term toxicity onBruchydunio rerio. The effect concentration (EC50 was considered to be less the 100 mg/l.

2.Test material was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

 

10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 12 mg/l . Toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >12 mg/l

Before death, fish tended to become very slow moving at higher concentration. Dead fish were dyed on heads and fins . Surviving fish were dyed dark green in concentration above 1mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 12 -100 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for short term toxicity of fish as " acute Aquatic 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the concentration range 12 -100mg/L .