Adenosine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September 2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

not applicable

Principles of method if other than guideline:

Respirometric degradation and toxicity test (ready biodegradability)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Adenosine

EC Number:

200-389-9

EC Name:

Adenosine

Cas Number:

58-61-7

Molecular formula:

C10H13N5O4

IUPAC Name:

adenosine

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source: ARA Reinach (5 times washed)
- Concentration of sludge: 30 mg/L dry matter

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 89.8 mg/L

Based on:

other: nominal test item concentration based on 100 mg ThOD/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST SYSTEM
- Test performed in triplicate (three test flasks)

CONTROL AND BLANK SYSTEM
BL (blank)
NaB – BL (Natriumbenzoate minus blank)
TES- BL (test item minus blank)
TOX – TES (toxicity control)
PHY (sterile control)
TES- BL (NO2, NO3 determined)

MEASUREMENTS
Samples were taken every 6 h.

Reference substance

Reference substance:

other: Sodium benzoate CAS 532-32-1

Results and discussion

Preliminary study:

none

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 100

Sampling time:

28 d

Any other information on results incl. tables

Results after 28 d, mean values

Vessel	mg/l O2	± mg/l
BL (blank)	32.5	3.5
NaB – BL (sodium benzoate minus blank)	158.2	4.8
TES- BL (test item minus blank)	204.5	12.6
TOX – TES (toxicity control)	131.4	1.2
PHY (sterile control)	3.4	0.3
TES- BL (NO2, NO3 determined)	204.5	12.6

 

The degradation curve obtained with the reference substance confirmed the suitability of inoculum and test conditions.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

A 28-d ready biodegradability test (Respirometry Test similar to guideline OECD 301 F) using activated sludge of a municipal sewage treatment plant indicated that adenosine (CAS 58-61-7) reached 100% degradation. The pass level of 60% for ready biodegradability as defined in test guideline OECD 301 was reached within the 10-d window and, therefore, the substance can be termed as readily biodegradable.

Executive summary:

A 28-d ready biodegradability test (Respirometry Test similar to guideline OECD 301 F) using activated sludge of a municipal sewage treatment plant indicated that adenosine (CAS 58-61-7) reached 100% degradation. The pass level of 60% for ready biodegradability as defined in test guideline OECD 301 was reached within the 10-d window and, therefore, the substance can be termed as readily biodegradable.